The Comparison of Antioxidative/Oxidative Profile in Blood, Colostrum and Milk of Early Post-partum Cows and Their Newborns
The Comparison of Antioxidative/Oxidative Profile in Blood, Colostrum and Milk of Early Post-partum Cows and Their Newborns
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DOI：
10.1111/j.1439-0531.2010.01737.x
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发表时间：
2011-10-01
影响因子：
1.7
通讯作者：
Kankofer, M.
中科院分区：
文献类型：
--
作者：
Albera, E.;Kankofer, M.
分娩行为不仅与女性而且与新生儿的抗氧化/氧化失衡有关。该研究的目的是比较产后早期奶牛及其新生儿血液、初乳和牛奶中的抗氧化/氧化特征。二十五头健康怀孕奶牛及其新生儿被纳入研究。产后立即、产后 24、48 小时以及产后 6 和 12 天采集血液和初乳/乳样本。通过分光光度法测定抗氧化防御参数，包括总抗氧化能力（TAC）和谷胱甘肽过氧化物酶（GSH-Px）以及脂质（LPI）和蛋白质（SH）过氧化强度参数。获得的结果表明实验期间检查参数值的动态变化（在产后 24 至 48 小时内显着）以及母亲与新生儿之间的关系。除 GSH-Px 外，新生儿血液中的所有参数均显着高于其母亲。此外，初乳中的TAC和GSH-Px在前24小时内与母亲及其新生儿的血液中一样较低，随后表现出相反的关系。母亲及其新生儿在出生后 48 小时内的初乳中 LPI 较高，而初乳中的 SH 较低，而受检动物的血浆中 SH 较低。检测时间内抗氧化参数值显着升高，而SH基团含量呈下降趋势，脂质过氧化强度呈波动趋势。检查参数变化的动态可能表明抗氧化/氧化状态的暂时不平衡以及生物体对当前活性氧过度产生造成的挑战的充分反应。本研究可能为奶牛和犊牛产后和产后期的研究带来新的知识，并可能有助于预防该时期的疾病。
Act of delivery is supposed to be connected with antioxidative/oxidative imbalance not only in female but also in her newborn. The aim of the study was to compare antioxidative/oxidative profile of cows and their newborns in blood, colostrum and milk during early post-partum period. Twenty-five healthy pregnant cows and their newborns were included into study. Blood and colostrum/milk samples were obtained immediately after parturition, 24, 48 h as well as 6 and 12 days post-partum. Parameters of antioxidative defence including total antioxidant capacity (TAC) and glutathione peroxidase (GSH-Px) as well as parameters of lipid (LPI) and protein (SH) peroxidation intensity were determined spectrophotometrically. Obtained results indicated dynamic changes in values of examined parameters during time of experiment, which were significantly marked between 24 and 48 h postpartum, and the relationship between mother and her newborn. All parameters except for GSH-Px were markedly higher in blood of newborns as in their mothers. Moreover, TAC and GSH-Px in colostrum were lower as in blood of mothers and their newborns during first 24 h and showed opposite relationship later on. LPI was higher in colostrum as in plasma of mothers and their newborns during first 48 h, while SH was lower in colostrum as in plasma of examined animals. Values of antioxidative parameters increased significantly within examined period of time, while the content of SH groups tended to decrease and lipid peroxidation intensity fluctuated. Dynamics in changes of examined parameters may suggest temporary imbalance in antioxidative/oxidative status and adequate answer of organism to current challenge resulting from overproduction of reactive oxygen species. Present study may bring new knowledge into studies of post-parturient and post-natal period in cows and calves and may help preventing from diseases of this period.