Sucrose represses the developmentally controlled transient activation of the plastocyanin gene in Arabidopsis thaliana seedlings
Sucrose represses the developmentally controlled transient activation of the plastocyanin gene in Arabidopsis thaliana seedlings
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DOI：
10.1104/pp.110.2.455
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发表时间：
1996-02-01
期刊：
影响因子：
7.4
通讯作者：
Smeekens, SCM
中科院分区：
文献类型：
--
作者：
Dijkwel, PP;Kock, PAM;Smeekens, SCM
拟南芥（Arabidopsis thaliana）质体青素（plasocyanin， PC）基因在幼苗发育早期不受光照的影响而被激活。在黄化幼苗中，PC mRNA水平瞬间升高，在黑暗中生长2 d后达到最大水平。在携带嵌合pc -启动子：荧光素酶融合基因的黄化转基因幼苗中，荧光素酶活性在生长2天后同样增加。蔗糖可抑制pcmrna和荧光素酶活性的瞬时升高。非代谢糖和聚乙二醇对PC基因表达没有主要影响。此外，光照下生长的幼苗也表现出与光照下生长的幼苗相似的PC mRNA水平和荧光素酶活性的瞬时和蔗糖敏感性增加，但光照下生长的幼苗的表达水平高出15倍。这些发现表明存在一种蔗糖敏感的、发育控制的表达机制，该机制独立于光的作用。
The plastocyanin (PC) gene of Arabidopsis thaliana is activated independently of light during early seedling development. In etiolated seedlings, PC mRNA levels increase transiently and a maximum dark level is reached after 2 d of growth in darkness. In etiolated transgenic seedlings carrying a chimeric PC-promoter: luciferase fusion gene, luciferase activity is similarly increased after 2 d of growth. The transient increase in PC mRNA and luciferase activity levels can be repressed by sucrose. Nonmetabolizable sugars and polyethylene glycol do not have a major effect on PC gene expression. Also, light-grown seedlings show a similar transient and sucrose-sensitive increase in PC mRNA levels and luciferase activity, as in dark-grown seedlings, but here expression levels are 15-fold higher. These findings suggest the presence of a sucrose-sensitive, developmentally controlled expression mechanism that operates independently of light.