Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases
Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases
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DOI：
10.3343/alm.2012.32.5.359
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发表时间：
2012-09-01
影响因子：
4.9
通讯作者：
Hong, Seong Geun
中科院分区：
文献类型：
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作者：
Hong, Sang Sook;Kim, Kyeongmi;Hong, Seong Geun
近年来，在韩国有越来越多的产KPC肺炎克雷伯菌的报道。改良Hodge试验可用作产A类碳青霉烯酶（CAC）临床分离株的表型筛选试验;然而，它不能区分碳青霉烯酶类型。CAC类型的确认对于确保最佳治疗和预防传播非常重要。本研究应用一种新的多重PCR方法在单一反应中检测和区分CAC基因。设计了4对引物，用于扩增编码4个CAC家族（SME、IMI/NMC-A、KPC和GES）的片段。多重PCR检测了4个CAC家族的所有检测基因，这些基因可以根据基因类型通过片段大小进行区分。这种多重PCR为检测和区分不产金属β-内酰胺酶的碳青霉烯类耐药菌株中的CAC基因提供了一种简单而有用的方法。
In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-beta-lactamase nonproducers.