In vivo interaction of nucleophosmin/B23 and protein C23 during cell cycle progression in HeLa cells
In vivo interaction of nucleophosmin/B23 and protein C23 during cell cycle progression in HeLa cells
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DOI：
10.1016/s0304-3835(99)00184-6
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发表时间：
1999-09-20
期刊：
影响因子：
9.7
通讯作者：
Yung, BYM
中科院分区：
文献类型：
--
作者：
Liu, HT;Yung, BYM
通过使用交联剂，数字信号处理器，努力鉴定与核磷蛋白/B23相互作用的蛋白(S)。核磷蛋白/B23和蛋白C23单抗均能识别约140 kDa的交联型蛋白复合体。从C23单抗免疫沉淀的交联物中可以检测到C23和核磷蛋白/B23。虽然在间期和胞质分裂中观察到核磷蛋白/B23和蛋白C23的关联，但在早中期和中期细胞中没有检测到这种关联。在放线菌素D诱导的细胞生长抑制过程中，核磷蛋白/B23和C23与C23的相互作用不仅存在于核磷蛋白/B23和C23主要定位于核仁的细胞中，而且还存在于核磷蛋白/B23和C23从核仁移位到核质的细胞中。纯化的重组GST-B23经前中期细胞提取液(诺可达唑滞留细胞)或cdc2激酶磷酸化后，仍可与C23免疫共沉淀。因此，核磷蛋白/B23在有丝分裂过程中不与C23相互作用的事实不能简单地用核磷蛋白/B23的有丝分裂磷酸化来解释。我们的发现为进一步阐明核磷蛋白/B33和蛋白C23在细胞周期进程和细胞生长中的作用提供了一些可能性。(C)1999爱思唯尔爱尔兰科学有限公司。保留所有权利。
By using the cross-linking reagent, DSP, efforts were made to identify the protein(s) that interact with nucleophosmin/B23. A cross-linked protein complex at molecular weight of about 140 kDa was recognized by both nucleophosmin/B23 and protein C23 MAbs. Both C23 and nucleophosmin/B23 could be detected from the cross-linked complex immunoprecipitated by C23 MAb. The association between nucleophosmin/B23 and protein C23 while being observed at interphase and cytokinesis, was not detected in prometaphase and metaphase cells. interactions of nucleophosmin/B23 with C23 not only could be found in cells in which nucleophosmin/B23 and C23 were both mainly localized to the nucleolus, but also in cells in which nucleophosmin/B23 and C23 had translocated from the nucleolus to the nucleoplasm during actinomycin D-induced cell growth inhibition. The purified recombinant GST-B23 being phosphorylated by prometaphase cell extracts (nocodazole-arrested cells) or cdc2 kinase could still bt: co-immunoprecipitated with C23. Consequently, the fact that nucleophosmin/B23 did not interact with C23 during mitosis could not be explained simply by mitotic phosphorylation of nucleophosmin/B23. Our findings suggest some possibilities for further elucidation of the actions of nucleophosmin/B33 and protein C23 in cell cycle progression and cell growth. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.