DRISEE overestimates errors in metagenomic sequencing data.
DRISEE overestimates errors in metagenomic sequencing data.
复制标题
DOI:
10.1093/bib/bbt010
复制
发表时间:
2014-09
影响因子:
9.5
通讯作者:
Sogin ML
中科院分区:
文献类型:
--
作者:
Eren AM;Morrison HG;Huse SM;Sogin ML
The extremely high error rates reported by Keegan et al. in ‘A platform-independent method for detecting errors in metagenomic sequencing data: DRISEE’ (PLoS Comput Biol 2012;8:e1002541) for many next-generation sequencing datasets prompted us to re-examine their results. Our analysis reveals that the presence of conserved artificial sequences, e.g. Illumina adapters, and other naturally occurring sequence motifs accounts for most of the reported errors. We conclude that DRISEE reports inflated levels of sequencing error, particularly for Illumina data. Tools offered for evaluating large datasets need scrupulous review before they are implemented.
登录
查看更多内容
影响因子:
3
作者:
Rosen MJ;Callahan BJ;Fisher DS;Holmes SP
通讯作者:
Holmes SP
影响因子:
12.3
作者:
Huse SM;Huber JA;Morrison HG;Sogin ML;Welch DM
通讯作者:
Welch DM
影响因子:
1.7
作者:
Shi, Haixiang;Schmidt, Bertil;Mueller-Wittig, Wolfgang
通讯作者:
Mueller-Wittig, Wolfgang
影响因子:
3.7
作者:
Schröder J;Bailey J;Conway T;Zobel J
通讯作者:
Zobel J
影响因子:
1.7
作者:
Zhao, Xiaohong;Palmer, Lance E.;Wittenberg, Gayle M.
通讯作者:
Wittenberg, Gayle M.