An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach
An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach
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DOI：
10.1007/s11356-017-9367-9
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发表时间：
2018-04-01
影响因子：
5.8
通讯作者：
Pugazhendhi, Arivalagan
中科院分区：
文献类型：
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作者：
Shanmuganathan, Rajasree;MubarakAli, Davoodbasha;Pugazhendhi, Arivalagan
在纳米粒子合成的各种方法中，银纳米粒子（AgNPs）的生物合成由于其多功能性而受到极大关注。本研究以胡萝卜提取物为原料合成银纳米粒子，并将头孢曲松与银纳米粒子偶联，以提高其抗菌效果。采用紫外-可见光谱、傅立叶变换红外光谱、原子力显微镜、动态光散射和透射电镜等手段对所制备的AgNPs进行了表征，发现其表面等离子体共振峰位于420 nm处，纳米粒子呈球形，粒径约为20 nm。测试了未缀合的AgNP和头孢曲松缀合的AgNP对头孢曲松耐药的人类病原体、蜡状芽孢杆菌、金黄色葡萄球菌、肺炎克雷伯氏菌和铜绿假单胞菌的抗微生物功效。在50 μ g/mL的浓度下，与未缀合的AgNP（18 mm）相比，与头孢曲松缀合的AgNP显示出更高的抑制作用（23 mm）。发现未缀合的和头孢曲松缀合的AgNP在50 μ g/mL下对EAC细胞均无毒。随着AgNPs浓度的增加，观察到剂量依赖性的细胞毒活性。头孢曲松结合的银纳米粒子比未结合的银纳米粒子显示出更高的活性。增强的活性可用于治疗头孢曲松耐药的人类病原体。
Of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (AgNPs) received great attention due to their versatile properties. In this report, Daucus carota extract was used for the synthesis of AgNPs and ceftriaxone was conjugated with AgNPs to enhance their antimicrobial efficacy. The conjugated and unconjugated AgNPs were characterized by adopting UV-Vis spectroscopy, FTIR, AFM, DLS, and TEM, which revealed the SPR peak at 420 nm and spherical shaped nanoparticles of 20 nm size, respectively. The antimicrobial efficacies of the unconjugated AgNPs and ceftriaxone-conjugated AgNPs were tested against ceftriaxone-resistant human pathogens, Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The ceftriaxone-conjugated AgNPs showed high inhibitory action (23 mm) than the unconjugated AgNPs (18 mm) at the concentration of 50 mu g/mL. Both the unconjugated and ceftriaxone-conjugated AgNPs were found to be non-toxic on EAC cells at 50 mu g/mL. The dose-dependent cytotoxic activities were observed on increasing the concentration of the AgNPs. The ceftriaxone-conjugated AgNPs showed high activity than the unconjugated AgNPs. The enhanced activity could be useful to treat ceftriaxone-resistant human pathogens.