Systematic analysis of SNARE localization in the filamentous fungus Aspergillus oryzae
Systematic analysis of SNARE localization in the filamentous fungus Aspergillus oryzae
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DOI：
10.1016/j.fgb.2007.04.012
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发表时间：
2007-12-01
影响因子：
3
通讯作者：
Kitamoto, Katsuhiko
中科院分区：
文献类型：
--
作者：
Kuratsu, Masahiro;Taura, Ayako;Kitamoto, Katsuhiko
尽管它们在应用生物学和基础生物学上都非常重要，但丝状真菌中囊泡运输的研究迄今为止非常有限。在这里，我们确定了21个基因，这可能是一个总的集合，编码推定的陷阱蛋白，囊泡运输的关键因素，利用丝状真菌曲霉菌的全基因组序列。随后的系统分析，以确定使用EGFP融合的嵌合体定位的假定的SNARE显示，大多数假定的SNARE显示出类似的亚细胞分布，其对应的芽殖酵母。然而，A.例如SNARE定位在隔膜处/附近以及存在明显非冗余的质膜Qa-SNARE。总的来说，这一分析使我们能够提供一个概述泡运输和细胞器分布在A。米。(c)2007爱思唯尔公司All rights reserved.
In spite of their great importance for both applied and basic biology, studies on vesicular trafficking in filamentous fungi have been so far very limited. Here, we identified 21 genes, which might be a total set, encoding putative SNARE proteins that are key factors for vesicular trafficking, taking advantage of available whole genome sequence in the filamentous fungus Aspergillus oryzae. The subsequent systematic analysis to determine the localization of putative SNAREs using EGFP-fused chimeras revealed that most putative SNAREs show similar subcellular distribution to their counterparts in the budding yeast. However, there existed some characteristic features of SNAREs in A. oryzae, such as SNARE localization at/near the septum and the presence of apparently non-redundant plasma membrane Qa-SNAREs. Overall, this analysis allowed us to provide an overview of vesicular trafficking and organelle distribution in A. oryzae. (c) 2007 Elsevier Inc. All rights reserved.