Effects of exercise and insulin on mitogen-activated protein kinase signaling pathways in rat skeletal muscle
Effects of exercise and insulin on mitogen-activated protein kinase signaling pathways in rat skeletal muscle
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DOI：
10.1152/ajpendo.1996.271.2.e403
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发表时间：
1996-08-01
影响因子：
5.1
通讯作者：
Moller, DE
中科院分区：
文献类型：
--
作者：
Goodyear, LJ;Chang, PY;Moller, DE
在哺乳动物细胞中的研究已经确定存在至少三种不同的丝裂原活化蛋白激酶（MAP激酶）信号通路，它们可被多种生长因子和/或环境应激物激活。我们研究了身体运动（一种生理应激物）以及胰岛素（一种代谢刺激剂和生长因子）是否会激活大鼠骨骼肌中的c - jun NH₂末端激酶（JNK）、p38激酶和/或细胞外调节激酶（ERK；p42(MAPK)和p44(MAPK)）信号通路。动物在电动跑步机上跑步10 - 60分钟（20米/分钟，10%坡度）后立即进行研究，或者在腹腔注射胰岛素（20单位/只大鼠）后5 - 30分钟进行研究。在整个研究的时间过程中，运动使骨骼肌JNK活性增加了2到3倍，而胰岛素并没有显著增加JNK活性。运动对p38活性有轻微刺激，胰岛素则没有。通过核糖体S6激酶 - 2活性测定和磷酸化特异性p42(MAPK)/p44(MAPK)免疫印迹评估，ERK激酶通路可被运动和胰岛素激活。这些数据首次证明运动可刺激骨骼肌中的多种细胞内信号通路。这些MAP激酶信号通路的激活可能介导骨骼肌生长和代谢对运动的响应变化。
Studies in mammalian cells have established the existence of at least three distinct mitogen-activated protein kinase (MAP kinase) signaling pathways that are activated by a variety of growth factors and/or environmental stressors. We determined whether physical exercise, a physiological stressor, and insulin, a metabolic stimulator and growth factor, activate the c-jun NH2-terminus kinase (JNK), the p38 kinase, and/or the extracellular regulatory kinases (ERK; p42(MAPK) and p44(MAPK)) signaling pathways in rat skeletal muscle. Animals were studied immediately after running on a motorized treadmill for 10-60 min (20 m/min, 10% grade) or 5-30 min after an intraperitoneal injection of insulin (20 U/rat). Exercise increased skeletal muscle JNK activity by two- to threefold throughout the time course studied, whereas insulin did not significantly increase JNK activity. The p38 activity was slightly stimulated by exercise and not by insulin. The ERK kinase pathway, as assessed by ribosomal S6 kinase-2 activity assays and phosphospecific p42(MAPK)/p44(MAPK) immunoblotting, was stimulated by both exercise and insulin. These data are the first demonstration of exercise stimulating multiple intracellular signaling pathways in skeletal muscle. Activation of these MAP kinase signaling pathways may mediate changes in skeletal muscle growth and metabolism that occur in response to exercise.