Chloroplast biogenesis during the early stage of leaf development in rice
Chloroplast biogenesis during the early stage of leaf development in rice
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DOI：
10.5511/plantbiotechnology.27.85
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发表时间：
2010-01-01
影响因子：
1.6
通讯作者：
Iba, Koh
中科院分区：
文献类型：
--
作者：
Kusumi, Kensuke;Chono, Yoko;Iba, Koh
水稻成叶发育过程可分为7个时期(P0~P6期)。在叶片伸长和基本叶片结构建立的P4期，研究了叶片发育过程中叶绿体的生物发生和生理变化。叶片中的叶绿素含量在P4早期几乎可以忽略不计，进入P4后期后迅速增加。叶绿素荧光比(Fv/Fm)也显著增加，最终值与成熟叶片相当。基因表达分析表明，在P4期，叶绿体经历了三个分化阶段：(1)叶绿体分裂和DNA复制；(2)叶绿体遗传系统的建立；(3)光合器官的激活。这些观察表明，P4是叶片发育的关键，在此期间，叶片在形态和生理上都迅速分化，P4叶片适合于研究叶绿体和叶片发育之间的生理关系。
In rice, the developmental process in leaf formation can be divided into 7 stages (stages P0 to P6). We investigated chloroplast biogenesis and physiological changes in the developing leaves at the stage P4, during which leaf blade elongation and establishment of basic leaf blade structure occur. Chlorophyll content was negligible in the leaves early in the P4 stage and increased rapidly as they enters the late P4 phase. Chlorophyll fluorescence ratio (Fv/Fm) also increased markedly and the final value was comparable with that of mature leaves. Gene expression analysis showed that during the P4 stage, chloroplasts in the leaf cell undergo all three steps of differentiation: (i) plastid division and DNA replication (ii) establishment of plastid genetic system (iii) activation of photosynthetic apparatus. These observations suggest that the P4 is key in the development of a leaf, during which leaf rapidly differentiated both morphologically and physiologically, and that the P4 leaf is suitable for investigation of physiological relationships between chloroplast and leaf development.