Intercalated cushion cells within the cardiac outflow tract are derived from the myocardial troponin T type 2 (Tnnt2) Cre lineage.
Intercalated cushion cells within the cardiac outflow tract are derived from the myocardial troponin T type 2 (Tnnt2) Cre lineage.
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DOI：
10.1002/dvdy.24641
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Kern CB
中科院分区：
文献类型：
--
作者：
Mifflin JJ;Dupuis LE;Alcala NE;Russell LG;Kern CB
发展成肺动脉瓣（PV）前尖和主动脉瓣（AV）非冠状尖的夹层垫的起源尚不清楚。Cre转基因与Rosa TdTomato-EGFP报告基因组合用于在闰垫发育期间产生AV和PV尖瓣的三维谱系映射。Tie2-Cre; EGFP用于标记内皮源性间充质细胞，Wnt 1-Cre;EGFP用于标记心脏神经嵴和心肌肌钙蛋白T（Tnnt 2）Cre;EGFP用于标记心肌谱系。在胚胎12.5天（E12.5）时，最高百分比的闰垫细胞为Tnnt 2-Cre; EGFP阳性，PV为68.0%，AV为50.0%。Tnnt 2 mRNA和Tnnt 2-Cre蛋白在闰垫中均不表达，Tnnt 2-Cre系闰垫细胞间充质标志物Sox 9和versican也呈阳性。Tnnt 2-Cre谱系存在于E11.5形成的闰垫内，并存在于闰垫衍生的PV和AV尖，并在出生后0天定位于纤维层。发育中的流出道的夹层垫填充有Tnnt 2-Cre衍生的细胞，这是一种Cre报告基因，以前用于追踪和切除心肌，以前与间充质细胞无关。
The origin of the intercalated cushions that develop into the anterior cusp of the pulmonary valve (PV) and the noncoronary cusp of the aortic valve (AV) is not well understood. Cre transgenes in combination with the Rosa TdTomato-EGFP reporter were utilized to generate three-dimensional lineage mapping of AV and PV cusps during intercalated cushion development. Tie2-Cre;EGFP was used to mark endothelial-derived mesenchymal cells, Wnt1-Cre;EGFP for cardiac neural crest and cardiac Troponin T (Tnnt2)Cre;EGFP, for myocardial lineage. The highest percentage of intercalated cushion cells at embryonic day 12.5 (E12.5) was Tnnt2-Cre; EGFP positive; 68.0% for the PV and 50.0% AV. Neither Tnnt2 mRNA nor Tnnt2-Cre protein was expressed in the intercalated cushions; and the Tnnt2-Cre lineage intercalated cushion cells were also positive for the mesenchymal markers Sox9 and, versican. Tnnt2-Cre lineage was present within the forming intercalated cushions from E11.5 and was present in the intercalated cushion derived PV and AV cusps and localized to the fibrosa layer at postnatal day 0. Intercalated cushions of the developing outflow tract are populated with Tnnt2-Cre derived cells, a Cre reporter previously utilized for tracing and excision of the myocardial and not previously associated with mesenchymal cells.
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影响因子：
2.7
作者：
Dupuis LE;McCulloch DR;McGarity JD;Bahan A;Wessels A;Weber D;Diminich AM;Nelson CM;Apte SS;Kern CB
通讯作者：
Kern CB
影响因子：
2.5
作者：
Christoffels, VM;Hoogaars, WMH;Campione, M
通讯作者：
Campione, M
影响因子：
4.6
作者：
Galli, Daniela;Dominguez, Jorge N.;Buckingham, Margaret E.
通讯作者：
Buckingham, Margaret E.
影响因子：
6
作者：
Nandakumar, KS;Svensson, L;Holmdahl, R
通讯作者：
Holmdahl, R
影响因子：
2.5
作者：
Kern, Christine B.;Norris, Russell A.;Mjaatvedt, Corey H.
通讯作者：
Mjaatvedt, Corey H.