Modeling of ion processes in atmospheric pressure matrix-assisted laser desorption/ionisation.
Modeling of ion processes in atmospheric pressure matrix-assisted laser desorption/ionisation.
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DOI：
10.1002/rcm.3061
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发表时间：
2007-07
期刊：
影响因子：
--
通讯作者：
V. Berkout;S. Kryuchkov;V. Doroshenko
中科院分区：
文献类型：
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作者：
V. Berkout;S. Kryuchkov;V. Doroshenko
常压基质辅助激光解吸/电离（AP-MALDI）是生物分子质谱分析中一种方便、快速的离子生成方法。这种技术，像其他大气压力电离方法一样，在离子从大气传输到质谱仪的真空中遭受离子损失。本文描述了一个描述离子形成和离子向质谱仪入口毛细管运动的简单模型。采用边界元法（BEM）对MALDI板附近的气流和电场进行了数值计算。离子随气体流动而运动，并根据离子迁移特性在电场中漂移。该模型中离子信号对电场强度的依赖性与实验结果吻合良好。
Atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) has proven a convenient and rapid method for ion production in the mass spectrometric analysis of biomolecules. This technique, like other atmospheric pressure ionization methods, suffers from ion loss during ion transmission from the atmosphere into the vacuum of the mass spectrometer. In this work, a simple model describing ion formation and ion motion towards the inlet capillary of the mass spectrometer is described. Both the gas flow and electric field near the MALDI plate were numerically calculated using the boundary element method (BEM). The ions were moving along with the gas flow and drifting in the electric field in accordance with their ion mobility properties. The ion signal dependence on an electric field strength obtained in the proposed model correlates well with experimental results.