THE RNA POLYMERASE-II MOLECULE AT THE 5' END OF THE UNINDUCED HSP70 GENE OF DROSOPHILA-MELANOGASTER IS TRANSCRIPTIONALLY ENGAGED
THE RNA POLYMERASE-II MOLECULE AT THE 5' END OF THE UNINDUCED HSP70 GENE OF DROSOPHILA-MELANOGASTER IS TRANSCRIPTIONALLY ENGAGED
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DOI：
10.1016/s0092-8674(88)91087-2
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发表时间：
1988-09-09
期刊：
影响因子：
64.5
通讯作者：
LIS, JT
中科院分区：
文献类型：
--
作者：
ROUGVIE, AE;LIS, JT
培养的果蝇细胞的蛋白质-DNA交联已经表明，在体内，在诱导热休克之前，大约有一个RNA聚合酶I分子与主要热休克基因hsp 70的启动子区相关。在这里，我们表明，这种启动子相关的RNA聚合酶II分子的转录参与，并已形成一个新生的RNA链的长度约25个核苷酸，但显然是在这一点上被捕，无法进一步渗透到热休克蛋白70基因没有热诱导。检测后启动RNA聚合酶复合物的启动子区的失活基因表明，有一个转录控制机制，作为一个步骤，在转录延长早期。
Protein-DNA cross-linking of cultured Drosophila cells has shown that, in vivo, prior to the induction of heat shock, there is approximately one molecule of RNA polymerase I associated with the promoter region of the major heat shock gene, hsp70. Here, we show that this promoter-associated RNA polymerase II molecule is transcriptionally engaged and has formed a nascent RNA chain of approximately 25 nucleotides in length, but is apparently arrested at that point and unable to penetrate further into the hsp70 gene without heat induction. The detection of post-initiation RNA polymerase complex on the promoter region of the inactive gene suggests that there is a transcriptional control mechanism that acts as a step early in transcript elongation.