Mixture modeling of microarray gene expression data.
Mixture modeling of microarray gene expression data.
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DOI：
10.1186/1753-6561-1-s1-s50
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发表时间：
2007
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影响因子：
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通讯作者：
Finch SJ
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文献类型：
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作者：
Yang Y;Tashman AP;Lee JY;Yoon S;Mao W;Ahn K;Kim W;Mendell NR;Gordon D;Finch SJ
大约28%的基因似乎具有遵循混合分布的表达模式。我们使用一阶和二阶偏相关系数来识别三个和四个与性别无关的基因，这些基因高度相关，而且也是混合体。我们鉴定了18个三重奏和35个四重奏的混合物，并评价了它们的混合物分布一致性。一致性被定义为基于其贝叶斯后验概率同时落入具有较高平均值的分量或同时落入具有较低平均值的分量的观测值的比例。这些三重奏和四重奏的符合率都在80%以上。这三重奏和四重奏中涉及33个基因。用VARIMAX旋转的因子分析根据它们的因子负荷确定了三个基因组。其中一组18个基因的符合率为56.7%，另一组8个基因的符合率为60.8%，第三组7个基因的符合率为69.6%。这些比率中的每一个都非常显著，这表明这些基因的混合机制可能有强大的生物学基础。贝叶斯因子筛选通过鉴定与第一组中最一致的五个基因的表达表型显著相关的六个单核苷酸多态来证实这一假设。
About 28% of genes appear to have an expression pattern that follows a mixture distribution. We use first- and second-order partial correlation coefficients to identify trios and quartets of non-sex-linked genes that are highly associated and that are also mixtures. We identified 18 trio and 35 quartet mixtures and evaluated their mixture distribution concordance. Concordance was defined as the proportion of observations that simultaneously fall in the component with the higher mean or simultaneously in the component with the lower mean based on their Bayesian posterior probabilities. These trios and quartets have a concordance rate greater than 80%. There are 33 genes involved in these trios and quartets. A factor analysis with varimax rotation identifies three gene groups based on their factor loadings. One group of 18 genes has a concordance rate of 56.7%, another group of 8 genes has a concordance rate of 60.8%, and a third group of 7 genes has a concordance rate of 69.6%. Each of these rates is highly significant, suggesting that there may be strong biological underpinnings for the mixture mechanisms of these genes. Bayesian factor screening confirms this hypothesis by identifying six single-nucleotide polymorphisms that are significantly associated with the expression phenotypes of the five most concordant genes in the first group.