ATTENUATION PHENOTYPE OF A CELL CULTURE-ADAPTED VARIANT OF HEPATITIS-A VIRUS (HM175/P16) IN SUSCEPTIBLE NEW-WORLD OWL MONKEYS
ATTENUATION PHENOTYPE OF A CELL CULTURE-ADAPTED VARIANT OF HEPATITIS-A VIRUS (HM175/P16) IN SUSCEPTIBLE NEW-WORLD OWL MONKEYS
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DOI：
10.1093/infdis/168.3.592
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发表时间：
1993-09-01
影响因子：
6.4
通讯作者：
LEMON, SM
中科院分区：
文献类型：
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作者：
TAYLOR, KL;MURPHY, PC;LEMON, SM
在4只血清反应阴性的猫头鹰猴中，用2.8 × 10（4）个放射免疫形成单位的病毒静脉接种，评估了克隆分离的细胞培养适应性甲型肝炎病毒（HM 175/p16）的毒力。该病毒是高度减毒的，尽管其完整的核苷酸序列仅包含来自野生型基因组的19个突变。只有3只猴子产生了甲型肝炎病毒抗体（只有2只在病毒接种后96天内）。1只猴出现病毒血症和血清转氨酶水平显著升高。在该动物中，接种后30-33天出现最大病毒血症和粪便散毒。相比之下，在相关细胞培养适应但仍具有肝毒性的病毒（HM 175/S18）的早期研究中，6只动物中记录到病毒血症，静脉接种约十分之一病毒后18或19天出现病毒血症峰值和粪便脱落病毒。
The virulence of a clonally isolated, cell culture-adapted hepatitis A virus (HM175/p16) was assessed in 4 seronegative owl monkeys inoculated intravenously with 2.8 X 10(4) radioimmunofocus-forming units of virus. The virus was highly attenuated, even though its complete nucleotide sequence contains only 19 mutations from the wild-type genome. Only 3 monkeys developed antibodies to hepatitis A virus (only 2 within 96 days of virus inoculation). One monkey had viremia and significantly elevated serum aminotransferase levels. In this animal, maximum viremia and fecal shedding of virus occurred 30-33 days after inoculation. In contrast, in earlier studies of a related cell culture-adapted but still hepatovirulent virus (HM175/S18), viremia was documented in 6 of 6 animals and peak viremia and fecal shedding of virus occurred 18 or 19 days after intravenous inoculation of about one-tenth as much virus.