Functional gene transfer from intracellular bacteria to mammalian cells
Functional gene transfer from intracellular bacteria to mammalian cells
复制标题
DOI：
10.1038/nbt0998-862
复制
发表时间：
1998-09-01
影响因子：
46.9
通讯作者：
Courvalin, P
中科院分区：
文献类型：
--
作者：
Grillot-Courvalin, C;Goussard, S;Courvalin, P
我们提供了从细菌到哺乳动物细胞的功能DNA直接转移的证据。通过克隆假结核耶尔森菌的入侵基因而获得的侵袭性大肠杆菌K12二氨基乳酸型营养缺陷菌，通过简单的共孵育将DNA转移到多种哺乳动物细胞系中。单核增生李斯特菌李斯特菌溶菌素基因的共表达提高了部分细胞的转移效率。获得性基因的表达发生在分裂和静止细胞中。细菌将遗传物质转移到非专业吞噬细胞和巨噬细胞的唯一要求是能够侵入宿主细胞。
We provide evidence of direct transfer of functional DNA from bacteria to mammalian cells. An Escherichia coli K12 diaminopimelate auxotroph made invasive by cloning the invasin gene from Yersinia pseudotuberculosis transfers DNA after simple co-incubation, into a variety of mammalian cell lines. Transfer efficiency was enhanced in some cells by coexpression of the gene for listeriolysin from Listeria monocytogenes. Expression of the acquired genes occurs in both dividing and quiescent cells. The only requirement for bacteria to transfer genetic material into nonprofessional phagocytic cells and macrophages is the ability to invade the host cell.