Neuronal expression of brain derived neurotrophic factor in the injured telencephalon of adult zebrafish
Neuronal expression of brain derived neurotrophic factor in the injured telencephalon of adult zebrafish
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DOI：
10.1002/cne.24352
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发表时间：
2018-03-01
影响因子：
2.5
通讯作者：
Lucini, Carla
中科院分区：
文献类型：
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作者：
Cacialli, Pietro;D'angelo, Livia;Lucini, Carla
在动物界，中枢神经系统的修复能力差异很大。在哺乳动物的大脑中，再生机制非常有限，新形成的神经元不能存活更长时间，可能是因为不适合的局部环境。相反，鱼可以在损伤后修复大脑，使受损区域快速完全恢复。斑马鱼是一种被广泛用作脊椎动物模型的硬骨鱼，其脑在损伤后也具有很高的再生能力。利用这一相关模型，本研究的目的是探讨脑源性神经营养因子(BDNF)在端脑刺伤后再生能力中的作用。脑源性神经营养因子参与多种脑功能，在创伤性脑损伤后的修复过程中发挥关键作用。已有研究表明，BDNF可增强神经前体细胞的增殖活性，促进神经元向损伤区域迁移，并因其抗细胞凋亡作用而显示出存活特性。用定量聚合酶链式反应和原位杂交法检测损伤后1、4、7和15天的端脑组织中BDNF基因表达水平，发现损伤端脑组织中BDNF基因表达水平在损伤后突然升高。原位杂交和免疫细胞化学双重染色显示，BDNF mRNA仅限于成熟神经元细胞。脑源性神经营养因子mRNA表达的神经元主要集中在损伤周围区域，在损伤后1d达高峰。综上所述，这些结果突出了脑源性神经营养因子在大脑修复过程中的作用，并加强了斑马鱼在再生神经发生研究中的价值。
The reparative ability of the central nervous system varies widely in the animal kingdom. In the mammalian brain, the regenerative mechanisms are very limited and newly formed neurons do not survive longer, probably due to a non-suitable local environment. On the opposite, fish can repair the brain after injury, with fast and complete recovery of damaged area. The brain of zebrafish, a teleost fish widely used as vertebrate model, also possesses high regenerative properties after injury. Taking advantage of this relevant model, the aim of the present study was to investigate the role of brain-derived neurotrophic factor (BDNF) in the regenerative ability of adult brain, after stab wound telencephalic injury. BDNF is involved in many brain functions and plays key roles in the repair process after traumatic brain lesions. It has been reported that BDNF strengthens the proliferative activity of neuronal precursor cells, facilitates the neuronal migration toward injured areas, and shows survival properties due to its anti-apoptotic effects. BDNF mRNA levels, assessed by quantitative PCR and in situ hybridization at 1, 4, 7, and 15 days after the lesion, were increased in the damaged telencephalon, mostly suddenly after the lesion. Double staining using in situ hybridization and immunocytochemistry revealed that BDNF mRNA was restricted to cells identified as mature neurons. BDNF mRNA expressing neurons mostly increased in the area around the lesion, showing a peak 1 day after the lesion. Taken together, these results highlight the role of BDNF in brain repair processes and reinforce the value of zebrafish for the study of regenerative neurogenesis.