Release of natriuretic factor from rat heart-lung preparation by atrial distension.
Release of natriuretic factor from rat heart-lung preparation by atrial distension.
复制标题
通过心房扩张从大鼠心肺制剂中释放钠尿因子。
DOI：
10.1152/ajpregu.1984.247.6.r1093
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Dietz,JR
中科院分区：
文献类型：
--
作者：
Dietz,JR
使用离体大鼠心肺制剂检验了血容量增加导致心房舒张释放心房钠尿因子 (ANF) 的假设。心肺标本用氟碳乳液灌注两次，每次 20 分钟。在第一阶段，静脉回流储液器放置在右心房上方 1-2 厘米的位置[低中心静脉压（低 CVP）]，第二阶段放置在心脏上方 7-12 厘米的位置（高 CVP）。收集并保存每个时期使用的灌注液，以供以后使用麻醉大鼠作为生物测定来分析利尿钠活性。将低或高 CVP 灌注液样本注入测定大鼠的腹主动脉，同时从膀胱导管收集尿液。以 0.5 ml/min 的速率输注高 CVP 灌注液 3 分钟，导致尿流量从 10 微升/分钟增加至 28 微升/分钟（P 小于 0.01），钠排泄从 0.14 mueq/min 增加至 1.34 mueq/min（P 小于 0.01），钾排泄从 0.17 mueq/min 增加至 0.81 mueq/min（P 小于 0.01）。 0.01）。低CVP灌注液的输注未能产生显着的利尿、尿钠排泄或血钾排泄。这些结果与 ANF 可能是一种参与控制血容量的激素的假设是一致的。
The hypothesis that an increase in blood volume results in the release of an atrial natriuretic factor (ANF) via atrial stretch was examined using an isolated rat heart-lung preparation. The heart lung preparation was perfused with a fluorocarbon emulsion for two periods of 20 min. During the first period the venous return reservoir was placed at a level of 1–2 cm above the right atrium [low central venous pressure (low CVP)] and at a level 7–12 cm above the heart (high CVP) for the second period. The perfusate used for each period was collected and saved for later analysis of natriuretic activity using anesthetized rats as a bioassay. Samples of either low or high CVP perfusate were infused into the abdominal aorta of the assay rats while urine was collected from a bladder catheter. A 3-min infusion of the high CVP perfusate at a rate of 0.5 ml/min resulted in an increase in urine flow from 10 to 28 microliter/min (P less than 0.01), sodium excretion from 0.14 to 1.34 mueq/min (P less than 0.01), and potassium excretion from 0.17 to 0.81 mueq/min (P less than 0.01). Infusion of the low CVP perfusate failed to produce a significant diuresis, natriuresis, or kaliuresis. These results are consistent with the hypothesis that ANF may be a hormone involved in the control of blood volume.