Exercise induces interleukin-8 receptor (CXCR2) expression in human skeletal muscle
Exercise induces interleukin-8 receptor (CXCR2) expression in human skeletal muscle
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DOI：
10.1113/expphysiol.2006.034769
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发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
Pedersen, Bente Klarlund
中科院分区：
文献类型：
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作者：
Frydelund-Larsen, Lone;Penkowa, Milena;Pedersen, Bente Klarlund
运动诱导骨骼肌纤维内白细胞介素-8（IL-8）mRNA和蛋白表达的显著增加。白细胞介素-8属于含有Glu-Leu-Arg（ELR）基序的CXC趋化因子亚家族。具有ELR基序的CXC趋化因子是体内有效的血管生成因子，并且IL-8已显示通过结合CXC受体2（CXCR 2）在人微血管内皮细胞中充当血管生成因子。在本研究中，我们研究了白细胞介素-8受体CXCR2的表达在人类骨骼肌活检后向心性运动。健康志愿者被随机分配到在60%最大摄氧量下进行3小时的自行车测力计运动（n = 8）或休息（n = 7）。在运动前（0 h）、运动后即刻（3 h）、运动后4.5、6、9和24 h从股外侧肌获取肌肉活检样本。与运动前样本相比，运动（3和4.5小时）后骨骼肌CXCR2 mRNA显著增加。运动前和运动结束时（3小时），骨骼肌活检组织中CXCR2蛋白的表达较低。然而，在4.5 - 9小时，通过免疫组织化学测定，在血管内皮中观察到CXCR2蛋白的增加，并且在肌纤维中也观察到轻微的增加。本研究表明，同心圆运动诱导CXCR2 mRNA和蛋白质表达的血管内皮细胞的肌纤维。这些发现表明，肌源性IL-8可能通过CXCR2受体信号传导局部刺激血管生成。
Exercise induces a marked increase in interleukin-8 (IL-8) mRNA and protein expression within skeletal muscle fibres. Interleukin-8 belongs to a subfamily of CXC chemokines containing a Glu-Leu-Arg (ELR) motif. CXC chemokines with ELR motifs are potent angiogenic factors in vivo, and IL-8 has been shown to act as an angiogenic factor in human microvascular endothelial cells by binding to the CXC receptor 2 (CXCR2). In the present study, we examined the expression of the interleukin-8 receptor CXCR2 in human skeletal muscle biopsies after concentric exercise. Healthy volunteers were randomized to either 3 h of cycle ergometer exercise at 60% of maximum oxygen uptake (n = 8) or rest (n = 7). Muscle biopsy samples were obtained from the vastus lateralis before exercise (0 h), immediately after exercise (3 h), and at 4.5, 6, 9 and 24 h. Skeletal muscle CXCR2 mRNA increased significantly in response to exercise (3 and 4.5 h) when compared with pre-exercise samples. Expression of the CXCR2 protein was low in skeletal muscle biopsies before exercise and at the end of the exercise period (3 h). However, at 4.5-9 h, an increase in CXCR2 protein was seen in the vascular endothelium, and also slightly within the muscle fibres, as determined by immunohistochemistry. The present study demonstrates that concentric exercise induces CXCR2 mRNA and protein expression in the vascular endothelial cells of the muscle fibres. These findings suggest that muscle-derived IL-8 may act locally to stimulate angiogenesis through CXCR2 receptor signalling.