Herpesviruses in periodontal pocket and gingival tissue specimens
Herpesviruses in periodontal pocket and gingival tissue specimens
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DOI：
10.1034/j.1399-302x.2000.150103.x
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发表时间：
2000-02-01
影响因子：
--
通讯作者：
Slots, J
中科院分区：
文献类型：
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作者：
Contreras, A;Nowzari, H;Slots, J
人巨细胞病毒 (HCMV) 和 1 型 Epstein-Barr 病毒 (EBV-1) 经常在牙周袋深部的龈沟液中检测到，但关于牙龈组织中疱疹病毒的存在情况的信息很少或没有。这项研究研究了 11 个牙周健康部位和 14 个牙周炎部位的牙周袋和相应牙龈组织中疱疹病毒的存在情况。采用巢式聚合酶链式反应来鉴定每个测试样品中是否存在 HCMV、EBV-1、EBV-2、单纯疱疹病毒、人疱疹病毒 (HHV)-6、HHV-7 和 HHV-8。在健康的牙周部位，在 1 个（9%）牙龈组织样本中检测到 HCMV，在 2 个（18%）个袋样本中检测到 EBV-1，在 2 个（18%）牙龈组织样本中检测到 HCMV，在 3 个（27%）牙龈组织样本中检测到 EBV-1。在牙周炎病变中，在 9 个（64%）个牙袋样本和 12 个（86%）牙龈组织样本中检测到 HCMV，在 6 个（43%）个牙袋样本和 11 个（79%）牙龈组织样本中检测到 EBV-1。 HHV-6 和 HHV-8 仅在牙龈组织样本中检测到。目前的研究结果证实了牙周炎病变中频繁存在 HCMV 和 EBV-1，并建议使用牙龈组织标本检测牙周 HHV-6、HHV-7 和 HHV-8。
Human cytomegalovirus (HCMV) and Epstein-Barr virus type 1 (EBV-1) are frequently detected in crevicular fluid of deep periodontal pockets, but little or no information is available on occurence of herpesviruses in gingival tissue. This investigation studied the presence of herpesviruses in periodontal pockets and the corresponding gingival tissues from 11 periodontally healthy and 14 periodontitis sites. A nested-polymerase chain reaction was employed to identify the presence of HCMV, EBV-1, EBV-2, herpes simplex virus, human herpesvirus (HHV)-6, HHV-7 and HHV-8 in each test sample. In healthy periodontal sites, HCMV was detected in 1 (9%) and EBV-1 in 2 (18%) pocket samples, and HCMV was detected in 2 (18%) and EBV 1 in 3 (27%) gingival tissue samples. In periodontitis lesions, HCMV was detected in 9 (64%) pocket samples and in 12 (86%) gingival tissue samples, and EBV-1 was detected in 6 (43%) pocket samples and in 11 (79%) gingival tissue samples. HHV-6 and HHV-8 were detected exclusively in gingival tissue samples. The present findings confirm the frequent presence of HCMV and EBV-1 in periodontitis lesions and suggest using gingival tissue specimens for detecting periodontal HHV-6, HHV-7 and HHV-8.