Two different types of dehalogenases, LinA and LinB, involved in γ-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26 are localized in the periplasmic space without molecular processing
Two different types of dehalogenases, LinA and LinB, involved in γ-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26 are localized in the periplasmic space without molecular processing
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DOI：
10.1128/jb.181.17.5409-5413.1999
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发表时间：
1999-09-01
影响因子：
3.2
通讯作者：
Takagi, M
中科院分区：
文献类型：
--
作者：
Nagata, Y;Futamura, A;Takagi, M
γ-六氯环己烷（γ-HCH）是造成严重环境问题的几种高氯化杀虫剂之一。一种γ-HCH降解细菌，少动鞘氨醇单胞菌UT 26，在渗透压休克后，其细胞蛋白被分离成周质、胞质和膜组分。在UT 26中，两种不同类型的脱卤酶LinA（γ-六氯环己烷脱氯化氢酶）和LinB（1，3，4，6-四氯-1，4-环己二烯卤化物水解酶）参与了γ-六氯环己烷降解的早期步骤，它们中的大多数是在周质部分中检测到的，并且没有进行分子加工。此外，免疫电镜清楚地表明，LinA和LinB是周质蛋白。LinA和LinB都缺乏典型的输出信号序列，因此它们可能通过迄今未知的机制分泌到周质空间中。
gamma-Hexachlorocyclohexane (gamma-HCH) is one of several highly chlorinated insecticides that cause serious environmental problems. The cellular proteins of a gamma-HCH-degrading bacterium, Sphingomonas paucimobilis UT26, were fractionated into periplasmic, cytosolic, and membrane fractions after osmotic shock. Most of two different types of dehalogenase, LinA (gamma-hexachlorocyclohexane dehydrochlorinase) and LinB (1,3,4,6-tetrachloro-1,4-cyclohexadiene halidohydrolase), that are involved in the early steps of gamma-HCH degradation in UT26 was detected in the periplasmic fraction and had not undertaken molecular processing. Furthermore, immunoelectron microscopy clearly showed that LinA and LinB are periplasmic proteins. LinA and LinB both lack a typical signal sequence for export, so they may be secreted into the periplasmic space via a hitherto unknown mechanism.