Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells.
Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells.
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DOI：
10.1186/s13567-017-0433-6
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发表时间：
2017-05-04
影响因子：
4.4
通讯作者：
Salát J
中科院分区：
文献类型：
--
作者：
Kavanová L;Matiašková K;Levá L;Štěpánová H;Nedbalcová K;Matiašovic J;Faldyna M;Salát J
猪繁殖与呼吸综合征（PRRS）是影响全世界猪的最严重和经济上最重要的传染病之一，并且可能使猪容易遭受由例如细菌引起的继发细菌感染。副猪嗜血杆菌。本研究的目的是比较两种不同类型的巨噬细胞的敏感性，这些巨噬细胞在感染 PRRS 病毒 (PRRSV) 和同时感染副猪嗜血菌期间可能与两种病原体接触。肺泡巨噬细胞 (PAM) 作为常驻细胞，是抵御微生物入侵肺组织的第一道防线。另一方面，单核细胞衍生的巨噬细胞（MDM）代表炎症细胞在炎症部位积聚。虽然 PAM 对 PRRSV 引起的细胞病变作用相对具有抵抗力，但 MDM 对 PRRSV 感染更为敏感。感染 PRRSV 的 MDM 会增加促凋亡 Bad、Bax 和 p53 mRNA 的表达。 MDM 死亡率的增加也可能与感染 PRRSV 后产生更高强度的 ROS 相关。此外，单独感染副猪嗜血菌的MDM（但不是PAM）形成多核巨细胞（MGC）；在感染两种病原体的 MDM 中未观察到这些细胞。 MDM 对 PRRSV 感染的较高敏感性与有限的 MDM 存活和 MGC 形成限制有关，可能导致猪多因素呼吸道疾病的发生。本文的在线版本 (doi:10.1186/s13567-017-0433-6) 包含补充材料，可供授权用户使用。
Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant and economically important infectious diseases affecting swine worldwide and can predispose pigs to secondary bacterial infections caused by, e.g. Haemophilus parasuis. The aim of the presented study was to compare susceptibility of two different types of macrophages which could be in contact with both pathogens during infection with PRRS virus (PRRSV) and in co-infection with H. parasuis. Alveolar macrophages (PAMs) as resident cells provide one of the first lines of defence against microbes invading lung tissue. On the other hand, monocyte derived macrophages (MDMs) represent inflammatory cells accumulating at the site of inflammation. While PAMs were relatively resistant to cytopathogenic effect caused by PRRSV, MDMs were much more sensitive to PRRSV infection. MDMs infected with PRRSV increased expression of pro-apoptotic Bad, Bax and p53 mRNA. Increased mortality of MDMs may be also related to a higher intensity of ROS production after infection with PRRSV. In addition, MDMs (but not PAMs) infected with H. parasuis alone formed multinucleated giant cells (MGC); these cells were not observed in MDMs infected with both pathogens. Higher sensitivity of MDMs to PRRSV infection, which is associated with limited MDMs survival and restriction of MGC formation, could contribute to the development of multifactorial respiratory disease of swine. The online version of this article (doi:10.1186/s13567-017-0433-6) contains supplementary material, which is available to authorized users.