IDENTIFYING P-FACTORS IN DROSOPHILA BY MEANS OF CHROMOSOME BREAKAGE HOTSPOTS
IDENTIFYING P-FACTORS IN DROSOPHILA BY MEANS OF CHROMOSOME BREAKAGE HOTSPOTS
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DOI：
10.1016/0092-8674(81)90211-7
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
PRESTON, CR
中科院分区：
文献类型：
--
作者：
ENGELS, WR;PRESTON, CR
一种D.被称为杂种发育不全的黑腹动物被认为是由称为P因子的转座遗传因子引起的。这里提出的几条证据表明，至少有一些P因子的染色体位置可以被确定为频繁染色体断裂的点（热点）。从在X染色体上鉴定出4个热点的菌株（PI2）开始，当它们的染色体部分被来自已知缺乏P因子的菌株的同源部分取代时，各个热点消失。染色体上未被取代部分的所有热点都保持功能，表明它们可以自主行动。一个新的断裂位点，观察到一致的外观不稳定的突变在烧焦的刚毛位点（snw）。这种突变是由发育不良引起的，以前的遗传证据表明，它是由P因子在该位点的插入引起的。初步证据提出了在一定条件下的热点的位置的快速混乱，并描述了一种新的程序，用于有效地确定给定的染色体上的热点的位置。
A syndrome of germline abnormalities in D. melanogaster called hybrid dysgenesis is thought to be caused by transposable genetic elements known as P factors. Several lines of evidence presented here show that the chromosomal positions of at least some P factors can be identified as points of frequent chromosome breakage (hot-spots). Starting with a strain (.PI.2) in which 4 hotspots were identified on the X chromosome, individual hotspots vanished when their part of the chromosome was replaced by the homologous part from a strain known to lack P factors. All hotspots in the non-substituted parts of the chromosome remained functional, indicating that they can act autonomously. A new breakage site was observed coinciding with the appearance of an unstable mutation at the singed bristle locus (snw). This mutation was dysgenesis-induced, and previous genetic evidence suggested it was caused by the insertion of a P factor at that locus. Preliminary evidence is presented for rapid scrambling of the positions of hotspots under certain conditions, and a new procedure is described for efficiently determining the positions of hotspots on a given chromosome.