Genomic analysis of lncRNA and mRNA profiles in circulating exosomes of patients with rheumatic heart disease
Genomic analysis of lncRNA and mRNA profiles in circulating exosomes of patients with rheumatic heart disease
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DOI：
10.1242/bio.045633
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发表时间：
2019-12-01
期刊：
影响因子：
2.4
通讯作者：
Hu, Qinghua
中科院分区：
文献类型：
--
作者：
Luo, Yanli;Huang, Lingjin;Hu, Qinghua
风湿性心脏病（RHD）仍然是发展中国家最常见的心血管疾病之一。越来越多的证据表明，循环外泌体及其货物，包括mRNA和长链非编码RNA（lncRNA），在许多心血管疾病中发挥重要作用。然而，其在RHD中的具体作用仍未被探索。在本研究中，我们鉴定了231个lncRNA和179个mRNA，与健康对照相比，它们在从RHD患者收集的循环外泌体中差异表达。我们进行了基因本体（GO）和KEGG通路分析，并确定了五对lncRNA及其侧翼编码基因同时在循环外泌体中失调。总的来说，我们提供了第一个转录组分析，鉴定了RHD患者循环外泌体中差异表达的lncRNA和mRNA5，这可能为发现RHD的潜在生物标志物和治疗靶点带来有价值的见解。
Rheumatic heart disease (RHD) remains one of the most common cardiovascular conditions in developing countries. Accumulating evidence suggests that circulating exosomes and their cargoes, including mRNA and long noncoding RNA (lncRNA), play essential roles in many cardiovascular diseases. However, their specific roles in RHD remain unexplored. In the present study, we identified 231 lncRNAs and 179 mRNAs differentially expressed in the circulating exosomes harvested from RHD patients compared to healthy controls. We performed gene ontology (GO) and KEGG pathway analysis, and identified five pairs of lncRNAs and their flanking coding genes simultaneously dysregulated in the circulating exosomes. Collectively, we provide the first transcriptome analysis identifying differentially expressed lncRNAs and mRNA5 in circulating exosomes of RHD patients, which may bring valuable insights for the discovery of potential biomarkers and therapeutic targets for RHD.