Mechanism of nuclear movements in a multinucleated cell
Mechanism of nuclear movements in a multinucleated cell
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DOI：
10.1091/mbc.e16-11-0806
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发表时间：
2017-03-01
影响因子：
3.3
通讯作者：
Nedelec, Francois
中科院分区：
文献类型：
--
作者：
Gibeaux, Romain;Politi, Antonio Z.;Nedelec, Francois
多核细胞在许多生物体中是重要的，但是控制共享一个共同的细胞质的核的运动的机制还不清楚。在植物病原真菌棉阿舒囊菌的菌丝中，细胞核来回移动，偶尔会绕过彼此，从而阻止核簇的形成。这对遗传稳定性至关重要。这些运动依赖于从细胞核发出的细胞质微管，这些微管被锚定在皮层的动力蛋白马达拉动。利用三维随机模拟的参数约束的文献，我们预测皮质锚密度的核运动的特点。该模型占体内看到的复杂的核运动，使用一组最小的实验确定的成分。有趣的是，在芽殖酵母中，这些成分为后期纺锤体的振荡提供动力，但在A。gossypii中，该系统不限于特定的核循环阶段，可能是适应菌丝生长和多核性的结果。
Multinucleated cells are important in many organisms, but the mechanisms governing the movements of nuclei sharing a common cytoplasm are not understood. In the hyphae of the plant pathogenic fungus Ashbya gossypii, nuclei move back and forth, occasionally bypassing each other, preventing the formation of nuclear clusters. This is essential for genetic stability. These movements depend on cytoplasmic microtubules emanating from the nuclei that are pulled by dynein motors anchored at the cortex. Using three-dimensional stochastic simulations with parameters constrained by the literature, we predict the cortical anchor density from the characteristics of nuclear movements. The model accounts for the complex nuclear movements seen in vivo, using a minimal set of experimentally determined ingredients. Of interest, these ingredients power the oscillations of the anaphase spindle in budding yeast, but in A. gossypii, this system is not restricted to a specific nuclear cycle stage, possibly as a result of adaptation to hyphal growth and multinuclearity.