Plastin-3 is a diagnostic and prognostic marker for pancreatic adenocarcinoma and distinguishes from diffuse large B-cell lymphoma.
Plastin-3 is a diagnostic and prognostic marker for pancreatic adenocarcinoma and distinguishes from diffuse large B-cell lymphoma.
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DOI：
10.1186/s12935-021-02117-1
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发表时间：
2021-08-04
影响因子：
5.8
通讯作者：
Chen YJ
中科院分区：
文献类型：
--
作者：
Xiong F;Wu GH;Wang B;Chen YJ
Plastin-3（PLS 3;一种肌动蛋白结合蛋白）表达的改变与人类癌变相关，包括胰腺导管腺癌（PDA）。本研究首先评估差异表达基因（DEG），然后从生物信息学和实验上证实PLS 3能够预测PDA预后并将PDA与弥漫性大B细胞淋巴瘤区分开来。本研究筛选了多个在线数据库，并揭示了PDA、正常胰腺、弥漫性大B细胞淋巴瘤（DLBCL）和正常淋巴结组织中的DEG，然后集中于PLS 3。分析这些DEG的基因本体论（GO）术语，Kaplan-Meier曲线和对数秩检验，以表征其与PDA预后的相关性。绘制受试者工作特征曲线（ROC），并进行斯皮尔曼检验。通过逆转录定量聚合酶链反应（RT-qPCR）评估不同组织样本（n = 30）中的差异PLS 3表达。PDA与淋巴结、DLBCL、正常胰腺组织间存在大量DEG。5个DEG（NET 1、KCNK 1、MAL 2、PLS 1和PLS 3）与PDA患者的不良总生存率相关，但R2和ICGC数据集仅进一步验证了PLS 3。ROC分析显示PDA诊断的高PLS 3 AUC（曲线下面积）值，而PLS 3能够区分PDA与DLBCL。斯皮尔曼分析的结果显示，PLS 3表达与KRT 7、SPP 1和TGF β 1的水平相关。通过RT-qPCR进一步验证不同组织样本中的差异化PLS 3表达。PLS 3表达的改变有助于PDA的诊断、预后判断以及PDA与DLBCL的鉴别诊断。在线版本包含补充材料，可通过10.1186/s12935-021-02117-1获得。
Altered Plastin-3 (PLS3; an actin-binding protein) expression was associated with human carcinogenesis, including pancreatic ductal adenocarcinoma (PDA). This study first assessed differentially expressed genes (DEGs) and then bioinformatically and experimentally confirmed PLS3 to be able to predict PDA prognosis and distinguish PDA from diffuse large B-cell lymphoma. This study screened multiple online databases and revealed DEGs among PDA, normal pancreas, diffuse large B-cell lymphoma (DLBCL), and normal lymph node tissues and then focused on PLS3. These DEGs were analyzed for Gene Ontology (GO) terms, Kaplan–Meier curves, and the log-rank test to characterize their association with PDA prognosis. The receiver operating characteristic curve (ROC) was plotted, and Spearman’s tests were performed. Differential PLS3 expression in different tissue specimens (n = 30) was evaluated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). There were a great number of DEGs between PDA and lymph node, between PDA and DLBCL, and between PDA and normal pancreatic tissues. Five DEGs (NET1, KCNK1, MAL2, PLS1, and PLS3) were associated with poor overall survival of PDA patients, but only PLS3 was further verified by the R2 and ICGC datasets. The ROC analysis showed a high PLS3 AUC (area under the curve) value for PDA diagnosis, while PLS3 was able to distinguish PDA from DLBCL. The results of Spearman's analysis showed that PLS3 expression was associated with levels of KRT7, SPP1, and SPARC. Differential PLS3 expression in different tissue specimens was further validated by RT-qPCR. Altered PLS3 expression was useful in diagnosis and prognosis of PDA as well as to distinguish PDA from DLBCL. The online version contains supplementary material available at 10.1186/s12935-021-02117-1.
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影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
影响因子：
0.2
作者：
Ravindhran, Bharadhwaj;Prakash, Clement;Pavithra, B.
通讯作者：
Pavithra, B.
影响因子：
29.4
作者：
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影响因子：
64.8
作者：
Bailey, Peter;Chang, David K.;Grimmond, Sean M.
通讯作者：
Grimmond, Sean M.
DOI：
10.1073/pnas.1704961114
发表时间：
2017-09-19
影响因子：
11.1
作者：
Cohen, Joshua D.;Javed, Ammar A.;Lennon, Anne Marie
通讯作者：
Lennon, Anne Marie