In vivo intrinsic optical signal imaging of mouse retinas.
In vivo intrinsic optical signal imaging of mouse retinas.
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小鼠视网膜的体内固有光信号成像。
DOI：
10.1117/12.2212810
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Yao,Xincheng
中科院分区：
文献类型：
--
作者：
Wang,Benquan;Yao,Xincheng
固有光信号成像技术是一种很有前途的无创性眼科疾病诊断和研究方法。在追求临床应用之前，有必要使用动物模型进行更多的IOS研究，以确定IOS扭曲与眼部疾病之间的关系。大量的小鼠模型可用于研究IOS畸变与眼部疾病之间的关系。然而，小鼠视网膜的体内IOS成像是具有挑战性的，这是由于小的眼透镜（与青蛙眼睛相比）和不可避免的眼球运动。我们在此报告使用定制设计的功能OCT对小鼠视网膜进行vivoIOS成像。OCT系统提供小鼠视网膜的高分辨率（3 μm）和高速（高达500帧/秒）成像。使用配备有定制设计的耳杆和咬杆的动物保持器来最小化由于呼吸和心跳引起的眼球运动。OCT图像中的残留眼球运动通过精确的图像配准进一步补偿。动态OCT成像显示刺激递送后立即（<10 ms）来自感光器外节的快速IOS，并且与感光器IOS相比，还观察到来自视网膜内层的具有延迟时间过程的明确IOS变化。
Intrinsic optical signal (IOS) imaging is a promising noninvasive method for advanced study and diagnosis of eye diseases. Before pursuing clinical applications, more IOS studies employing animal models are necessary to establish the relationship between IOS distortions and eye diseases. Ample mouse models are available for investigating the relationship between IOS distortions and eye diseases. However,in vivoIOS imaging of mouse retinas is challenging due to the small ocular lens (compared to frog eyes) and inevitable eye movements. We report herein vivoIOS imaging of mouse retinas using a custom-designed functional OCT. The OCT system provided high resolution (3 μm) and high speed (up to 500 frames/s) imaging of mouse retinas. An animal holder equipped with a custom designed ear bar and bite bar was used to minimize eye movement due to breathing and heartbeats. Residual eye movement in OCT images was further compensated by accurate image registration. Dynamic OCT imaging revealed rapid IOSs from photoreceptor outer segments immediately (<10 ms) after the stimulation delivery, and unambiguous IOS changes were also observed from inner retinal layers with delayed time courses compared to that of photoreceptor IOSs.