Comparison of serum and salivary cotinine measurements by a sensitive high-performance liquid chromatography-tandem mass spectrometry method as an indicator of exposure to tobacco smoke among smokers and nonsmokers
Comparison of serum and salivary cotinine measurements by a sensitive high-performance liquid chromatography-tandem mass spectrometry method as an indicator of exposure to tobacco smoke among smokers and nonsmokers
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DOI：
10.1093/jat/24.5.333
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发表时间：
2000-07-01
影响因子：
2.5
通讯作者：
Pirkle, JL
中科院分区：
文献类型：
--
作者：
Bernert, JT;McGuffey, JE;Pirkle, JL
暴露于烟草烟雾，无论是主动吸烟和被动暴露于环境烟草烟雾，可以通过测量可替宁，尼古丁的代谢物，在各种生物来源，包括血液，尿液和唾液监测。以前，开发了一种灵敏的大气压电离串联质谱（LC-API-MS-MS）方法，用于血清中可替宁的测量，以支持一项大型的、经常性的国家流行病学调查。目前的研究检查了这种LC-API-MS-MS方法在200名健康成年人（包括吸烟者和非吸烟者）的血清和唾液可替宁测量中的应用。本研究的主要目的是评价血清和唾液可替宁浓度之间的关系，以促进使用唾液可替宁测量的流行病学研究结果与基于血清可替宁分析的现有国家数据之间的联系。结果表明，可以建立一种简单的线性关系来描述个体的血清和唾液可替宁浓度，并且描述这种关系的表达式可以用于估计具有合理准确度（约± 10%）的个体的血清可替宁浓度，给出他或她的唾液可替宁结果。进一步证实，唾液可替宁样本在采集后储存期间通常相当稳定，即使在环境温度下也是如此，并且该样本基质似乎非常适合许多流行病学调查的要求。
Exposure to tobacco smoke, both from active smoking and from passive exposure to environmental tobacco smoke, can be monitored by measuring cotinine, a melabolite of nicotine, in a variety of biological sources including blood, urine, and saliva. Previously, a sensitive atmospheric-pressure ionization, tandem mass spectrometric (LC-API-MS-MS) method for cotinine measurements in serum was developed in support of a large, recurrent national epidemiologic investigation. The current study examined the application of this LC-API-MS-MS method to both serum and saliva cotinine measurements in a group of 200 healthy adults, including both smokers and nonsmokers. The primary objective of this study was to evaluate the relationship between serum and saliva cotinine concentrations to facilitate the linking of results from epidemiologic studies using salivary cotinine measurements to existing national data based on serum cotinine analyses. The results indicate that a simple, linear relationship can be developed to describe serum and saliva cotinine concentrations in an individual, and the expression describing this relationship can be used to estimate with reasonable accuracy (approximately ± 10%) the serum cotinine concentration in an individual given his or her salivary cotinine result. It was further confirmed that saliva cotinine samples are generally quite stable during storage after collection, even at ambient temperatures, and this sample matrix appears to be well-suited to the requirements of many epidemiologic investigations.