Distribution of Fluorescence following Injection of Recombinant Adeno-Associated Virus Encoding Green Fluorescent Protein into the Paraventricular Nucleus
Distribution of Fluorescence following Injection of Recombinant Adeno-Associated Virus Encoding Green Fluorescent Protein into the Paraventricular Nucleus
复制标题
将编码绿色荧光蛋白的重组腺相关病毒注射到室旁核后的荧光分布
作者：
Helen L. Ward;J. Gardiner;W. M. Kong;K. Murphy;S. Bloom
我们利用重组2型腺相关病毒将编码绿色荧光蛋白的基因导入成年大鼠的中枢神经系统。通过荧光显微镜测定的基因表达不仅在注射部位观察到，而且在已知神经解剖学途径的轴突中也观察到。我们已经证实了增强的绿色荧光蛋白从下丘脑室旁核扩散到正中隆起和神经垂体。在注射侧对侧的室旁核中也可见含有增强的绿色荧光蛋白的细胞体。这些研究结果表明，重组腺相关病毒的基因转移可作为一种工具，调查下丘脑-垂体的相互作用，并在中枢神经系统的其他地方，跟踪轴突通路。
We have used recombinant type 2 adeno-associated virus to deliver the gene encoding green fluorescent protein into the central nervous system of adult rats. Gene expression, determined by fluorescent microscopy, was observed not only at the site of injection but also in axons following known neuroanatomical pathways. We have demonstrated a spread of enhanced green fluorescent protein from the paraventricular nucleus of the hypothalamus into the median eminence and neurohypophysis. Cell bodies containing enhanced green fluorescent protein were also visualized in the paraventricular nucleus contralateral to the side of injection. These findings suggest that gene transfer by recombinant adeno-associated virus could be used as a tool to investigate hypothalamic-pituitary interactions and, elsewhere in the central nervous system, to trace axonal pathways.
影响因子：
4.2
作者：
Bartlett, JS;Samulski, RJ;McCown, TJ
通讯作者：
McCown, TJ
腺病毒介导的基因递送至小鼠下丘脑大细胞神经元。
DOI：
10.1161/01.hyp.34.4.756
发表时间：
1999
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Vasquez,EC;Beltz,TG;Meyrelles,SS;Johnson,AK
通讯作者：
Johnson,AK