A second-generation integrated map of the silkworm reveals synteny and conserved gene order between lepidopteran insects
A second-generation integrated map of the silkworm reveals synteny and conserved gene order between lepidopteran insects
复制标题
DOI：
10.1534/genetics.106.055541
复制
发表时间：
2006-07-01
期刊：
影响因子：
3.3
通讯作者：
Sahara, Ken
中科院分区：
文献类型：
--
作者：
Yasukochi, Yuji;Ashakumary, Laksmikutty A.;Sahara, Ken
构建了家蚕第二代连锁图谱，重点定位了公共核苷酸数据库和细菌人工染色体（BAC）重叠群中出现的家蚕基因组序列。共874 BAC重叠群含有5067个克隆（22%的库），构建了基于PCR的筛选与序列标记位点（STS）来自全基因组鸟枪（WGS）序列。共有523个BAC重叠群，包括342个独立的基因注册在公共数据库和85个表达序列标签（EST），被放置到连锁图上。我们发现B之间存在明显的同线性和保守的基因顺序。mori和Heliconius melpomene在4个连锁群（LGs）中的遗传相似性，这强烈表明使用B.桑尺蠖作为鳞翅目昆虫比较基因组学研究的参考是非常可行的。
A second-generation linkage map was constructed for the silkworm, Bombyx mori, focusing on mapping Bombyx sequences appearing in public nucleotide databases and bacterial artificial chromosome (BAC) contigs. A total of 874 BAC contigs containing 5067 clones (22% of the library) were constructed by PCR-based screening with sequence-tagged sites (STSs) derived from whole-genome shotgun (WGS) sequences. A total of 523 BAC contigs, including 342 independent genes registered in public databases and 85 expressed sequence tags (ESTs), were placed onto the linkage map. We found significant synteny and conserved gene order between B. mori and a nymphalid butterfly, Heliconius melpomene, in four linkage groups (LGs), strongly suggesting that using B. mori as a reference for comparative genomics in Lepidotera is highly feasible.