SUPRAMOLECULAR ORGANIZATION OF GLYCOLYTIC-ENZYMES
SUPRAMOLECULAR ORGANIZATION OF GLYCOLYTIC-ENZYMES
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DOI：
10.1016/s0022-5193(85)80086-2
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发表时间：
1985-01-01
影响因子：
2
通讯作者：
MILMAN, LS
中科院分区：
文献类型：
--
作者：
KURGANOV, BI;SUGROBOVA, NP;MILMAN, LS
根据对糖酵解酶吸附到骨骼肌结构蛋白和红细胞膜上的数据、酶-酶相互作用的数据和细胞代谢对糖酵解酶活性的调节的数据的分析，提出了吸附到生物支持物上的糖酵解酶复合物的结构。在多酶复合物形成中起关键作用的是6-磷酸果糖激酶。该酶分子有两个结合位点，其中一个提供6-磷酸果糖激酶在支持物上的固定，另一个被果糖-1，6-二磷酸醛缩酶饱和。多酶复合物含有一个6-磷酸果糖激酶的四聚体分子和两个其他糖酵解酶的分子。己糖激酶不是复合物的一部分。多酶复合物的分子量约为2.6 × 104。106道尔顿。多酶复合物具有二级对称轴。多酶复合物的形成导致糖酵解过程的区室化。讨论了酶活性调节的物理化学机制（变构、解离和吸附机制）的整合问题。
On the basis of the analysis of the data on adsorption of glycolytic enzymes to structural proteins of skeletal muscles and to the erythrocyte membranes, the data on enzyme-enzyme interactions and the data on the regulation of activity of glycolytic enzymes by cellular metabolities, the structure of the glycolytic enzymes complex adsorbed to a biological support has been proposed. The key role in the formation of multienzyme complex belongs to 6-phosphofructokinase. The enzyme molecule has two association sites, one of which provides the fixation of 6-phosphofructokinase on the support and another is saturated by fructose-1,6-bisphosphate aldolase. The multienzyme complex contains one tetrameric molecule of 6-phosphofructokinase and two molecules of each of other glycolytic enzymes. Hexokinase is not a part of the complex. The molecular mass of the multienzyme complex is about 2.6 .times. 106 daltons. The multienzyme complex has symmetry axis of second order. The formation of the multienzyme complex leads to the compartmentation of glycolytic process. The problem of integration of physico-chemical mechanisms of enzyme activity regulation (allosteric, dissociative and adsorptive mechanisms) is discussed.