Rapid detection of polyethylene glycol sonolysis upon functionalization of carbon nanomaterials.
Rapid detection of polyethylene glycol sonolysis upon functionalization of carbon nanomaterials.
复制标题
DOI：
10.1177/1535370214567615
复制
发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Draper R
中科院分区：
文献类型：
--
作者：
Murali VS;Wang R;Mikoryak CA;Pantano P;Draper R
聚乙二醇及其相关聚合物经常用于碳纳米材料的功能化过程中，这涉及到超声波作用。然而，聚乙二醇对超声降解非常敏感，聚乙二醇降解产物对哺乳动物细胞有毒性。因此，必须评估潜在的聚乙二醇降解，以确保最终材料不包含可能会将人工制品引入实验结果的未记录污染物。本文介绍了一种检测聚乙二醇超声降解的简单、廉价的聚丙烯酰胺凝胶电泳法。该方法被用来监测不同超声时间后聚乙二醇磷脂结构和支链钉子的完整性。这种方法不仅有助于检测降解的聚乙二醇，而且还应有助于快速筛选超声波参数，以找到将聚乙二醇损害降至最低的最佳条件。
Polyethylene glycol (PEG) and related polymers are often used in the functionalization of carbon nanomaterials in procedures that involve sonication. However, PEG is very sensitive to sonolytic degradation and PEG degradation products can be toxic to mammalian cells. Thus, it is imperative to assess potential PEG degradation to ensure that the final material does not contain undocumented contaminants that can introduce artifacts into experimental results. Described here is a simple and inexpensive polyacrylamide gel electrophoresis method to detect the sonolytic degradation of PEG. The method was used to monitor the integrity of PEG phospholipid constructs and branched chain PEGs after different sonication times. This approach not only helps detect degraded PEG, but should also facilitate rapid screening of sonication parameters to find optimal conditions that minimize PEG damage.