Three-dimensional reconstruction of anomalous beige mouse macrophage lysosomes.
Three-dimensional reconstruction of anomalous beige mouse macrophage lysosomes.
复制标题
异常米色小鼠巨噬细胞溶酶体的三维重建。
DOI：
10.1002/jlb.46.5.441
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发表时间：
1989
影响因子：
5.5
通讯作者：
Sehgal,N
中科院分区：
文献类型：
--
作者：
Strausbauch,P;Sehgal,N
米色小鼠的细胞，Chediak-Higashi综合征的小鼠对应物，含有扩大的异常溶酶体。通过对一系列连续切片的电子显微照片的研究，结合计算机辅助分析，确定了这些来自腹腔巨噬细胞的溶酶体的三维结构。最常见的基本结构单元是双凹盘和空心杯/卵形结构。其他更奇异的形式是由这些基本的溶酶体变体融合而成的。在米色驻留和渗出的腹腔巨噬细胞中均发现异常溶酶体形式。对不携带米色突变的动物的溶酶体进行的类似研究显示存在较小但结构相似形式的基本溶酶体变体。后一个发现表明，米色动物中溶酶体变体本身的存在不是这些动物特有的发现，而是它们的独特性来自于它们的大小增加和相互融合的倾向。计算机辅助三维重建的有用性被证明。
Cells of the beige mouse, the murine counterpart of the Chediak-Higashi Syndrome, contain enlarged anomalous lysosomes. The three-dimensional structure of these lysosomes from peritoneal macrophage was ascertained by study of electron micrographs taken in a series of serial sections combined with computer-assisted analysis. The most common basic structural units identified were biconcave discs and hollow cup/ovoid-shaped structures. Other more bizarre forms were represented by the fusion of these basic lysosomal variants. Anomalous lysosomal forms were found in both beige resident and exudate peritoneal macrophage. A similar study of lysosomes in animals not carrying the beige mutation revealed the presence of smaller but structurally similar forms of the basic lysosomal variants. This latter finding indicates that the presence of lysosomal variants per se in the beige animal is not a finding unique to these animals but rather that their distinctiveness derives from their increased size and propensity to undergo fusion with each other. The usefulness of computer-assisted three-dimensional reconstruction is demonstrated.