Auxin-mediated cell cycle activation during early lateral root initiation
Auxin-mediated cell cycle activation during early lateral root initiation
复制标题
DOI：
10.1105/tpc.004960
复制
发表时间：
2002-10-01
期刊：
影响因子：
11.6
通讯作者：
Beeckman, T
中科院分区：
文献类型：
--
作者：
Himanen, K;Boucheron, E;Beeckman, T
侧根的形成可分为两个主要阶段：周鞘激活和分生组织建立。在拟南芥中，第一个侧根发生事件在空间和时间上是异步的，并且涉及木质部周壁中有限数量的细胞。为了研究侧根激活过程中的分子调控，我们建立了侧根诱导系统。用生长素运输抑制剂和外源生长素连续处理，以防止第一次形成分裂，然后激活整个周壁。我们的形态学和分子数据表明，在这个诱导系统中，木质部周壁激活同步和增强，以覆盖整个长度的根。该诱导系统可作为研究细胞周期同步再激活的一种新的植物体内系统。此外，Kip相关蛋白2（KRP 2）在周鞘中的表达模式及其异位表达数据显示，细胞周期蛋白依赖性激酶抑制剂在侧根发生的调节中起着重要作用。KRP 2似乎通过阻断G1-to-S转换来调节早期侧根的发生，并受生长素的转录调节。
Lateral root formation can be divided into two major phases: pericycle activation and meristem establishment. In Arabidopsis, the first lateral root initiation event is spatially and temporally asynchronous and involves a limited number of cells in the xylem pericycle. To study the molecular regulation during pericycle activation, we developed a lateral root-inducible system. Successive treatments with an auxin transport inhibitor and exogenous auxin were used to prevent the first formative divisions and then to activate the entire pericycle. Our morphological and molecular data show that, in this inducible system, xylem pericycle activation was synchronized and enhanced to cover the entire length of the root. The results also indicate that the inducible system can be considered a novel in planta system for the study of synchronized cell cycle reactivation. In addition, the expression patterns of Kip-Related Protein2 (KRP2) in the pericycle and its ectopic expression data revealed that the cyclin-dependent kinase inhibitor plays a significant role in the regulation of lateral root initiation. KRP2 appears to regulate early lateral root initiation by blocking the G1-to-S transition and to be regulated transcriptionally by auxin.