Structural, morphological and surface characteristics of two types of octacalcium phosphate-derived fluoride-containing apatitic calcium phosphates
Structural, morphological and surface characteristics of two types of octacalcium phosphate-derived fluoride-containing apatitic calcium phosphates
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DOI：
10.1016/j.actbio.2012.07.041
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发表时间：
2012-12-01
影响因子：
9.7
通讯作者：
Suzuki, O.
中科院分区：
文献类型：
--
作者：
Shiwaku, Y.;Anada, T.;Suzuki, O.
据报道，磷酸八钙(OCP)在水解成羟基磷灰石(HA)的过程中可以刺激骨再生。本研究旨在研究在12-230ppm氟化物(F)存在下，通过OCP水解法或直接沉淀法获得的含氟磷灰石钙磷酸盐(CAP)的结构、形态和表面性质。采用化学分析、X-射线衍射仪、场发射扫描电子显微镜、透射电子显微镜、选区电子衍射、傅立叶变换红外光谱、比表面积、溶解度、成骨活性和牛血清白蛋白(BSA)吸附等方法对产物进行了表征。X-射线衍射仪分析再次证实，两种制备方法都得到了较高F含量的磷灰石帽，但共沉淀产物(CF-CAP)保持了OCP的性质，特别是溶解性，而水解物(HF-CAP)则具有氟化磷灰石的特征。随着水解度的提高，片状OCP的结晶逐渐转变为棒状的CF帽和不规则的小HF帽。SAED分析检测到OCP和磷灰石晶体，甚至在最易降解的CF-帽中也是如此。小鼠骨髓基质ST-2细胞在CF-CAP上的生长情况好于HF-CAP。对牛血清白蛋白在HF-CAP上的吸附抑制作用强于在CF-CAP上。这些结果表明，OCP在水解过程中会产生不同的物理化学特征的磷灰石氟化帽，这取决于氟化物的引入途径，从而提供了具有生物意义的材料。(C)2012 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Octacalcium phosphate (OCP) has been reported to stimulate bone regeneration during hydrolysis into hydroxyapatite (HA). The present study was designed to characterize structural, morphological and surface properties of fluoride-containing apatitic calcium phosphates (CaP) obtained through OCP hydrolysis or direct precipitation of OCP in the presence of 12-230 ppm of fluoride (F). The products were characterized by chemical analysis, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and Fourier transform infrared spectroscopy (FTIR) as well as measurements of surface area, solubility, osteoblastic activities and bovine serum albumin (BSA) adsorption. XRD analysis re-confirmed that both preparations yielded more apatitic CaP with a higher concentration of F. However, the co-precipitated products (CF-CaP) maintained the properties of OCP, in particular the solubility, whereas the hydrolysis products (HF-CaP) had the characteristics of fluoridated apatite. The crystals of plate-like OCP were changed to the crystals of rod-like CF-CaP and small irregular HF-CaP with the advance of the hydrolysis. The SAED analysis detected both OCP and apatite crystals even in the most hydrolyzed CF-CaP. Mouse bone marrow stromal ST-2 cells grew better on CF-CaP compared with HF-CaP. BSA adsorption was inhibited on HF-CaP more than on CF-CaP. These results show that OCP produces physicochemically distinct apatitic fluoridated CaP during hydrolysis, regarding the structure, the crystal morphology and the protein adsorption, depending on the fluoride introduction route, which provides biologically interesting material. (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.