The extended application of The Rat Brain in Stereotaxic Coordinates in rats of various body weight
The extended application of The Rat Brain in Stereotaxic Coordinates in rats of various body weight
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大鼠大脑立体定位坐标在不同体重大鼠中的扩展应用。
DOI：
10.1016/j.jneumeth.2018.06.026
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发表时间：
2018-09-01
影响因子：
3
通讯作者：
Chen, Naihong
中科院分区：
文献类型：
--
作者：
Yang, Pengfei;Wang, Zhenzhen;Chen, Naihong
背景：在神经学研究中经常使用大鼠的立体定位术。常用的大鼠脑立体定位图集有科学上被引用次数第二多的著作《大鼠脑立体定位图》和分别以Wistar大鼠290g和SD大鼠225g为基础的大鼠脑立体定位图集。新方法：尽可能准确地测量大鼠的颅骨测量参数，并根据测量得到的曲线拟合方程，首次推导出不同体重大鼠的相对坐标。结果：如果将“标准”大鼠的靶区坐标定义为AP(A)、ML(B)、DV(C)，则相应的坐标AP(a‘)、ML(b’)、DV(C)，体重为m(G)的Wistar大鼠的Dv(c‘)为：A’=0.138xln(M)+0.177/0.138xln(290)+0.177 x ab‘=0.055xln(M)+0.515/0.055xln(290)+0.515 xBc’=0.054xln(M)+0.706/0.054xln(290)+0.706 x C-0.00012x(M-290)。：a‘=0.127xln(M)+0.176/0.127xln+0.176 x ab’=0.049xln(M)+0.556/0.049xln(225)+0.556 x bc‘=0.077xln(M)+0.604/0.077xln(225)+0.604 x c-0.00010x(m-225)单位，B，c，a‘，b’，c‘为厘米，m为单位为克。与现有方法的比较：在立体定位坐标下的大鼠脑适用于体重约290克的大鼠，而我们的公式将图谱的使用扩展到体重90-400克的大鼠。结论：这样可以扩大大鼠脑图谱的应用范围，结果将对立体定位操作有很大的帮助。
Background: Stereotaxic procedures in rats are frequently used in neurological research. The commonly used rat brain stereotaxic atlases are The Rat Brain in Stereotaxic Coordinates, the second most cited book in science, and Stereotaxic Atlas of Rat Brain (in Chinese) which based on Wistar rats of 290 g and SD rats of 225 g, respectively. However, rats of different weight are frequently used, while the stereotaxic coordinates of their brain regions show obvious differences with the rats on which the atlases were based.New method: The craniometric parameters were measured as accurately as possible, then curve fitting equations obtained from the parameters were used to deduce the relative coordinates in rats of different weight for the first time.Results: The results revealed that if the coordinates of target brain region were defined as AP(a), ML(b), DV(c) in "standard" rats, the corresponding coordinates AP(a'),ML(b'),DV(c') for rats weighingm(g) were described as follows:For Wistar rats using The Rat Brain in Stereotaxic Coordinates:a' = 0.138xln(m)+0.177/0.138xln(290)+0.177 x ab' = 0.055xln(m)+0.515/0.055xln(290)+0.515 x bc' = 0.054xln(m)+0.706/0.054xln(290)+0.706 x C-0.00012x(M-290)and for SD rats using Stereotaxic Atlas of Rat Brain(in Chinese):a' = 0.127xln(m)+0.176/0.127xln(225)+0.176 x ab' = 0.049xln(m)+0.556/ 0.049xln(225)+0.556 x bc' = 0.077xln(m)+0.604/0.077xln(225)+0.604 x c-0.00010x (m-225)Unit of a, b, c, a', b', c' are centimeter, unit of m is gram.Comparison with existing method: The Rat Brain in Stereotaxic Coordinates is applicable for rats weighing about 290 g, while our equations extended the use of atlas to rats weighing 90-400 g.Conclusions: In this manner, the application of the rat brain atlases could be extended and the results would be of great help in stereotaxic procedures.