Detection of viable Toxoplasma gondii in retail venison
Detection of viable Toxoplasma gondii in retail venison
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DOI：
10.1016/j.foodcont.2024.110354
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发表时间：
2024-02-06
期刊：
影响因子：
6
通讯作者：
Hamilton，C. M.
中科院分区：
文献类型：
--
作者：
Thomson，J.;Joeres，M.;Hamilton，C. M.
弓形虫是一种重要的人畜共患病原体，食用未煮熟的肉类是感染的重要危险因素。此前，一项对苏格兰零售肉类的小规模研究发现，T。gondiin鹿肉产品，但由于寄生虫的生存能力尚未确定，因此无法评估对公共卫生的风险。因此，本研究的目的是在这项工作的基础上建立。在零售鹿肉中的可食用性。从农场商店或超市购买23个鹿肉产品，并对50 g样品进行DNA提取和qPCR处理。所有T阳性的样本。在小鼠生物测定中评估gondiiby qPCR的活力。ViableT.在5份阳性鹿肉样品中，有2份（40%）分离到弓形虫。RFLP和微卫星分析的基因分型显示这两个菌株是II型。这是第一个报告viableT存在的研究。gondiiin零售鹿肉，并强调如果这种肉吃得不熟，对公众健康的潜在风险。消费者在食用鹿肉前应将其冷冻或彻底煮熟，以减少食源性弓形虫病的风险。
Toxoplasma gondiiis an important zoonotic pathogen and the consumption of undercooked meat is known to be a significant risk factor for infection. Previously, a small-scale study of retail meat in Scotland identified a high incidence ofT. gondiiin venison products but the risk to public health could not be assessed as parasite viability was not determined. The aim of the present study, therefore, was to build on this work and establishT. gondiiviability in retail venison. Twenty-three venison products were purchased from farm shops or supermarkets, and 50 g samples were processed for DNA extraction and qPCR. Any samples positive forT. gondiiby qPCR were assessed for viability in a mouse bioassay. ViableT. gondiiwas isolated from 2 out of 5 (40 %) positive venison samples. Genotyping by RFLP and microsatellite analysis revealed both isolates to be Type II. This is the first study to report the presence of viableT. gondiiin retail venison and highlights the potential risk to public health if this meat is eaten undercooked. Consumers should freeze venison, or cook it thoroughly, before consumption to reduce the risk of foodborne toxoplasmosis.