FGF-2 potently induces both proliferation and DSP expression in collagen type I gel cultures of adult incisor immature pulp cells
FGF-2 potently induces both proliferation and DSP expression in collagen type I gel cultures of adult incisor immature pulp cells
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DOI：
10.1016/j.bbrc.2004.10.136
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发表时间：
2004-12-17
影响因子：
3.1
通讯作者：
Tsuji, T
中科院分区：
文献类型：
--
作者：
Nakao, K;Itoh, M;Tsuji, T
我们研究了细胞因子和细胞外基质对未成熟大鼠切牙牙髓细胞增殖和分化的影响。这些未成熟的细胞在体外具有高增殖能力，不表达牙本质非胶原蛋白的mRNAs，如牙本质涎蛋白、骨涎蛋白和骨钙素，存在于成年大鼠切牙的根部。成纤维细胞生长因子-2刺激这些未成熟细胞的增殖，随后的矿化钙的产生是由β-甘油磷酸处理诱导的。此外，成纤维细胞生长因子-2显著诱导DSPmRNA和BSPmRNAs的表达，但仅限于I型胶原凝胶培养，而I型胶原板和纤维连接蛋白、层粘连蛋白或IV型胶原培养都不能产生这种作用，并产生足够的生理水平的这些转录本。尽管骨形态发生蛋白-4不能诱导未成熟牙髓细胞的增殖，也不能上调DSPmRNA的表达，但它与成纤维细胞生长因子-2一起对DSPs的转录水平有协同作用。这些结果表明，成纤维细胞生长因子-2的存在和I型胶原凝胶培养中未成熟牙髓细胞的三维形成对DSP的表达和成牙本质细胞的分化都是必不可少的。这些观察结果为研究成牙本质细胞系的定位和分化提供了有价值的信息，也为合理设计细胞因子和细胞外基质化合物用于新的牙科治疗中的再生治疗提供了基础。(C)2004 Elsevier Inc.保留所有权利。
We investigated the effects of both cytokines and extracellular matrices on the proliferation and differentiation of immature adult rat incisor dental pulp cells. These immature cells, which have a high-proliferative potency in vitro and do not express mRNAs for dentin non-collagenous proteins such as dentin sialoprotein (DSP), bone sialoprotein (BSP), and osteocalcin, exist in the root regions of adult rat incisors. Fibroblast growth factor-2 (FGF-2) stimulated the proliferation of these immature cells and the subsequent production of mineralized calcium was induced by beta-glycerophosphate treatment. Additionally, FGF-2 dramatically induced the expression of DSP and BSP mRNAs, but only in collagen type I gel cultures, whereas neither plate-coated collagen type I nor fibronectin, laminin or collagen type IV cultures could produce this effect and generate sufficient physiological levels of these transcripts. Although bone morphogenetic protein-4 could not induce the proliferation of immature dental pulp cells nor upregulate DSP mRNA expression, it had a synergistic effect upon DSP transcript levels in conjunction with FGF-2. These results suggest that both the presence of FGF-2 and the three-dimensional formation of immature dental pulp cells in collagen type I gel cultures are essential for both DSP expression and odontoblast differentiation. These observations provide valuable information concerning the study of the commitment and differentiation of odontoblast lineages, and also provide a basis for the rational design of cytokine and extracellular matrix based compounds for regenerative therapies in new dental treatments. (C) 2004 Elsevier Inc. All rights reserved.