Isolation and identification of glycosylphosphatidylinositol-anchored arabinogalactan proteins and novel β-glucosyl Yariv-reactive proteins from seeds of rice (Oryza sativa)
Isolation and identification of glycosylphosphatidylinositol-anchored arabinogalactan proteins and novel β-glucosyl Yariv-reactive proteins from seeds of rice (Oryza sativa)
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DOI：
10.1093/pcp/pch208
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发表时间：
2004-12-01
影响因子：
4.9
通讯作者：
Suzuki, Y
中科院分区：
文献类型：
--
作者：
Mashiguchi, K;Yamaguchi, I;Suzuki, Y
阿拉伯半乳聚糖蛋白（AGPs）是一类高度糖基化的胞外糖蛋白，在植物生长发育中起着重要作用。我们以前曾报道过的可能性，AGPs参与诱导α-淀粉酶的赤霉素（GA）在大麦糊粉层通过使用β-葡萄糖基Yariv试剂（β-GlcY），这已被假定为特异性结合AGPs。在本研究中，我们从富含糊粉细胞的米糠中分离出β-GlcY反应蛋白。经典的AGP和AG肽的N-末端序列从通过反相HPLC获得的亲水馏分中确定。有趣的是，一种新的非特异性脂质转移蛋白样蛋白（OsLTPL 1）和一种新的早期结瘤蛋白样蛋白（OsENODL 1）也被确定在更疏水的馏分从HPLC作为β-GlcY反应蛋白。进行编码这些蛋白质的基因的表达分析。经典的AGP、AG肽和OsLTPL 1在水稻的不同部位均有表达，而OsENODL 1在糊粉层表现出时空特异性表达。这种新的β-GlcY反应蛋白是一个有前途的候选人的GA行动在谷类种子的细胞外信号传导因子。此外，具有AG糖基化酶的蛋白质可能与β-GlcY反应的可能性可以拓宽AGP的定义。
Arabinogalactan proteins (AGPs) are highly glycosylated extracellular glycoproteins playing important roles in plant growth and development. We have previously reported the possibility that AGPs are involved in the induction of alpha-amylase by gibberellin (GA) in barley aleurone layers by using the beta-glucosyl Yariv reagent (beta-GlcY), which has been presumed to specifically bind AGPs. In this present study, we isolated beta-GlcY-reactive proteins from rice bran rich in aleurone cells. The N-terminal sequences of classical AGP and AG peptides were determined from hydrophilic fractions obtained by reversed phase HPLC. Interestingly, a novel non-specific lipid transfer protein-like protein (OsLTPL1) and a novel early nodulin-like protein (OsENODL1) were also identified in the more hydrophobic fractions from HPLC as beta-GlcY-reactive proteins. Expression analysis of the genes coding for these proteins was performed. While classical AGP, AG peptides and OsLTPL1 were expressed in various parts of rice, OsENODL1 showed temporally and spatially specific expression in the aleurone layers. This new beta-GlcY-reactive protein is a promising candidate for the extracellular signaling factors of GA action in cereal seeds. Furthermore, the possibility that proteins with the AG glycomodule might react with beta-GlcY may broaden the definition of AGPs.