Expression of claudin-7 and-8 along the mouse nephron
Expression of claudin-7 and-8 along the mouse nephron
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DOI：
10.1152/ajprenal.00384.2003
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发表时间：
2004-06-01
影响因子：
4.2
通讯作者：
Yu, ASL
中科院分区：
文献类型：
--
作者：
Li, WY;Huey, CL;Yu, ASL
紧密连接蛋白是紧密连接的膜蛋白，其决定上皮组织中细胞旁通透性的大小和选择性。哺乳动物肾小管不同节段的渗透性表现出相当大的异质性。为了确定密蛋白-7和-8的肾单位片段定位，使用亚型特异性抗体对小鼠肾脏切片进行免疫荧光染色。Claudin-8主要表达于沿着整个醛固酮敏感性远端肾单位的紧密连接处和长环状肾单位的细降支的晚期节段。这种表达模式与claudin-8作为细胞旁阳离子屏障的假定作用一致。相比之下，在与claudin-8相同的肾单位片段中发现了claudin-7，但其主要在基底外侧膜表达。
Claudins are integral membrane proteins of the tight junction that determine the magnitude and selectivity of paracellular permeability in epithelial tissues. The mammalian renal tubule exhibits considerable heterogeneity in the permeability properties of its different segments. To determine the nephron segment localization of claudin-7 and -8, immunofluorescence staining of mouse kidney sections was performed using isoform-specific antibodies. Claudin-8 was found to be expressed primarily at the tight junction along the entire aldosterone-sensitive distal nephron and in the late segments of the thin descending limbs of long-looped nephrons. This pattern of expression is consistent with the putative role of claudin-8 as a paracellular cation barrier. By contrast, claudin-7 was found in the same nephron segments as claudin-8, but it was expressed primarily at the basolateral membrane.