Central administration of melanin-concentrating hormone increases alcohol and sucrose/quinine intake in rats
Central administration of melanin-concentrating hormone increases alcohol and sucrose/quinine intake in rats
复制标题
DOI：
10.1097/01.alc.0000167741.42353.10
复制
发表时间：
2005-06-01
期刊：
影响因子：
--
通讯作者：
Woods, SC
中科院分区：
文献类型：
--
作者：
Duncan, EA;Proulx, K;Woods, SC
背景：酒精是一种热量化合物，有助于能量摄入。因此，调节能量平衡的肽可能会改变饮酒的动机。黑色素浓缩激素 (MCH) 调节能量稳态，并与影响饮酒的其他行为（即焦虑、体液平衡和奖励）有关。我们测试了这样的假设：MCH 会降低饮酒动机，进而减少焦虑。方法：使用蔗糖褪色技术训练大鼠饮用 10% 乙醇或等热量浓度的蔗糖。将MCH（1、5或10μg）或其盐水载体注入第三脑室（i3vt），并评估乙醇或蔗糖和食物的摄入量2小时。给予 i3vt MCH (10 μg)、神经肽 Y 或盐水后，在高架十字迷宫中对未接触过酒精的大鼠进行评估。结果：与假设相反，MCH 剂量依赖性增加酒精摄入量：盐水 = 0.7 +/- 0.1 g/kg，1 μg MCH = 1.0 +/- 0.1 g/kg，5 μg MCH = 1.2 +/- 0.1 g/kg，10 μg MCH = 1.8 +/- 0.3 g/kg (p < 0.01)，无论水是否存在，情况都是如此。同时可用或不可用。 MCH 还显着增加了蔗糖摄入量（盐水 = 1.0 +/- 0.3 g/kg，10 μg MCH = 1.4 +/- 0.5 g/kg；p < 0.05）。相对于生理盐水（58.2 +/- 23.8 秒），MCH 对张开手臂的时间没有影响（54.3 +/- 11.5 秒），而神经肽 Y（一种已知的抗焦虑药）增加了张开手臂的时间（119.2 +/- 22 秒，p < 0.05）。结论：我们得出结论，MCH 非特异性地增加摄取行为。此外，MCH 对焦虑没有明显影响。 MCH 增加酒精和/或蔗糖摄入量的能力可以通过 MCH 对能量平衡和/或奖励过程的影响来解释。
Background: Alcohol is a caloric compound that can contribute to energy intake. Therefore, peptides that regulate energy balance likely modify the motivation to consume alcohol. Melanin-concentrating hormone (MCH) regulates energy homeostasis and has been implicated in other behaviors that impact alcohol consumption (i.e., anxiety, fluid balance, and reward). We tested the hypothesis that MCH would decrease the motivation to consume alcohol secondarily to reducing anxiety., Methods: Rats were trained to drink 10% ethanol or an isocaloric concentration of sucrose with use of a sucrose-fading technique. MCH (1, 5, or 10 mu g) or its saline vehicle was administered into the third cerebral ventricle (i3vt), and intake of ethanol or sucrose and chow was assessed for 2 hr. Alcohol-naive rats were evaluated in an elevated plus maze after i3vt MCH (10 mu g), neuropeptide Y, or saline administration. Results: Contrary to the hypothesis, MCH dose-dependently increased alcohol intake: saline = 0.7 +/- 0.1 g/kg, 1 mu g MCH = 1.0 +/- 0.1 g/kg, 5 mu g MCH = 1.2 +/- 0.1 g/kg, and 10 mu g MCH = 1.8 +/- 0.3 g/kg (p < 0.01), and this was true whether water was simultaneously available or not. MCH also significantly increased sucrose intake (saline = 1.0 +/- 0.3 g/kg, 10 mu g MCH = 1.4 +/- 0.5 g/kg;p < 0.05). MCH had no effect on time spent in the open arms (54.3 +/- 11.5 see) relative to saline (58.2 +/- 23.8 sec), whereas neuropeptide Y, a known anxiolytic, increased time spent on the open arms (119.2 +/- 22 sec, p < 0.05). Conclusions: We conclude that MCH nonspecifically increases ingestive behavior. Furthermore, MCH had no apparent effect on anxiety. The ability of MCH to increase alcohol and/or sucrose intake may be explained by the effect of MCH on energy balance and/or reward processes.