ISOLATION OF MICROORGANISMS WITH LIGNIN TRANSFORMATION POTENTIAL FROM SOIL OF TENERIFE ISLAND
ISOLATION OF MICROORGANISMS WITH LIGNIN TRANSFORMATION POTENTIAL FROM SOIL OF TENERIFE ISLAND
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DOI：
10.1016/0038-0717(94)00174-y
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发表时间：
1995-02-01
影响因子：
9.7
通讯作者：
DELAFUENTE, G
中科院分区：
文献类型：
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作者：
FALCON, MA;RODRIGUEZ, A;DELAFUENTE, G
从加那利群岛特内里费岛铁铁质和铁铁质土壤中分离出44种真菌、15种放线菌和23种细菌，在以工业木质素聚合物组分为单一碳源的固体培养基上能够大量生长。44株真菌在木质素培养基上生长，22株桑德曼试验阳性，聚合物染料Poly R-478脱色。然而，在上述22株真菌中，只有青霉菌、尖孢镰刀菌、氧化拟盘菌、茄枯镰刀菌和一株担子菌真菌的过氧化物酶、Bavendamm、Nobles和漆酶平板试验呈阳性。这5种真菌中的每一种都代谢羟基化和非羟基化木质素相关的苯丙酸，无论是作为单一的C源还是在碳水化合物存在的情况下。这些真菌还能够分解代谢木质素模型松柏醇（DHP）的脱氢聚合物。（CO2）- c -14由c -14标记的环和c -14- β侧链标记的dhp进化而来。不完全真菌释放(CO2)-C-14的最大速率出现在这些真菌代谢的初始阶段。
A total of 44 fungi, 15 actinomycetes and 23 bacteria, isolated from ferrallitic and fersiallitic types of soil of Tenerife (Canary Islands) were capable of abundant growth on solid media supplemented with the polymeric fraction of industrial lignin as a single C source. Of 44 fungal strains growth on the media with lignin, 22 showed a positive Sundman's test and decolorization of polymeric dye Poly R-478. However, from the above 22 fungal strains, only Penicillium chrysogenum, Fusarium oxysporum, Pestalotia oxyanthi, Fusarium solani and a strain of basidiomycete fungus gave positive results for peroxidase, Bavendamm, Nobles, and laccase plate tests. Each of these 5 fungi metabolized hydroxylated and non-hydroxylated lignin-related phenylpropanoid acids, either as a single C source or in the presence of carbohydrate. These fungi were also capable of catabolizing lignin-model dehydrogenative polymerizate of coniferyl alcohol (DHP). (CO2)-C-14 evolving both from C-14-labelled ring- and C-14-beta-side-chain labelled-DHP. The maximum rate of (CO2)-C-14 release by fungi imperfecti appeared to be during the initial stages of the metabolism of these fungi.