Assembly in an in vitro splicing reaction of a mouse insulin messenger RNA precursor into a 60-40S ribonucleoprotein complex.
Assembly in an in vitro splicing reaction of a mouse insulin messenger RNA precursor into a 60-40S ribonucleoprotein complex.
复制标题
在体外剪接反应中将小鼠胰岛素信使 RNA 前体组装成 60-40S 核糖核蛋白复合物。
DOI：
10.1093/nar/14.9.3687
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发表时间：
1986
影响因子：
14.9
通讯作者：
Goldenberg,CJ
中科院分区：
文献类型：
--
作者：
Kaltwasser,G;Spitzer,SG;Goldenberg,CJ
含有两个外显子和一个内含子的SP 6/小鼠胰岛素RNA前体可以在从MOPC-315小鼠骨髓瘤细胞分离的部分纯化的核提取物中剪接。我们检测到了假定的RNA剪接中间体（内含子-3 '外显子）的lt 3形式，切除的内含子的lt 3形式，和mRNA剪接产物。凝胶纯化的RNA前体的体外剪接反应需要ATP和Mg 2+，并伴随着60- 40 S核糖核蛋白复合物的形成。60 S复合物的形成需要ATP。含有U1和U2 RNA的至少两个Sm snRNP是60- 40 S复合物的组分。这些snRNP的组装发生在剪接反应的早期，它需要ATP和含有内含子的前mRNA。
An SP6/mouse insulin RNA precursor containing two exons and one intron can be spliced in a partially purified nuclear extract isolated from MOPC-315 mouse myeloma cells. We have detected the putative RNA splicing intermediate (intron-3'exon) in a lariat form, the excised intron in a lariat form, and the mRNA spliced product. The in vitro splicing reaction of gel-purified RNA precursors requires ATP and Mg2plus;and was accompanied by the formation of a 60-40S ribonucleoprotein complex. The formation of the 60S complex requires ATP. At least two Sm snRNPs containing Ul and U2 RNAs are components of the 60-40S complex. The assemble of those snRNPs occurs early during the splicing reaction and it requires ATP and intron containing pre-mRNAs.