Setting a baseline for global urban virome surveillance in sewage
Setting a baseline for global urban virome surveillance in sewage
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DOI：
10.1038/s41598-020-69869-0
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发表时间：
2020-08-13
影响因子：
4.6
通讯作者：
Kwenda, Geofrey
中科院分区：
文献类型：
--
作者：
Nieuwenhuijse, David F.;Munnink, Bas B. Oude;Kwenda, Geofrey
特大城市的快速发展及其在世界各地日益紧密的联系正在成为新出现的疾病暴发的一个明显驱动因素。因此，早期发现异常疾病的出现和传播应包括这些城市，作为基于风险的监测的一部分。对城市污水进行全面的宏基因组测序有可能对社区中传播的病毒病原体的动态提供公正的见解，而不管是否获得护理，这一潜力已被证明可用于监测脊髓灰质炎病毒。在这里，我们从全球81个高密度城市地区的快照中详细描述了污水病毒组的特征，包括对潜在偏差的深入评估，作为全面病毒病原体监测的概念证明。我们展示了检测广泛的病毒和特定病毒群的地理和季节差异的能力。我们的研究结果为进一步研究城市污水样本的病毒监测提供了一个横截面基线，并将以前的研究置于全球视角。
The rapid development of megacities, and their growing connectedness across the world is becoming a distinct driver for emerging disease outbreaks. Early detection of unusual disease emergence and spread should therefore include such cities as part of risk-based surveillance. A catch-all metagenomic sequencing approach of urban sewage could potentially provide an unbiased insight into the dynamics of viral pathogens circulating in a community irrespective of access to care, a potential which already has been proven for the surveillance of poliovirus. Here, we present a detailed characterization of sewage viromes from a snapshot of 81 high density urban areas across the globe, including in-depth assessment of potential biases, as a proof of concept for catch-all viral pathogen surveillance. We show the ability to detect a wide range of viruses and geographical and seasonal differences for specific viral groups. Our findings offer a cross-sectional baseline for further research in viral surveillance from urban sewage samples and place previous studies in a global perspective.