A comparison of human umbilical cord matrix stem cells and temporomandibular joint condylar Chondrocytes for tissue engineering temporomandibular joint condylar cartilage
A comparison of human umbilical cord matrix stem cells and temporomandibular joint condylar Chondrocytes for tissue engineering temporomandibular joint condylar cartilage
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DOI：
10.1089/ten.2006.0150
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发表时间：
2007-08-01
影响因子：
--
通讯作者：
Detamore, Michael S.
中科院分区：
文献类型：
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作者：
Bailey, Mark M.;Wang, Limin;Detamore, Michael S.
颞下颌关节（TMJ）是现代肌肉骨骼医学中的一个难题。患有颞下颌关节紊乱病的患者通常会经历生活质量的重大损失，因为颞下颌关节紊乱病可能对日常活动造成的衰弱影响。软骨组织工程可以导致替代组织，可用于治疗颞下颌关节疾病。在这项研究中，使用转瓶6天的时间，以TMJ髁突软骨细胞或来源于人脐带基质（HUCM）的间充质样干细胞接种聚乙醇酸（PGA）支架。然后将样品在含有软骨形成因子的生长培养基或对照培养基中静态培养4周。4周后，HUCM构建体的免疫组织化学染色显示大量I型胶原蛋白和微量II型胶原蛋白的存在，而TMJ构建体显示几乎没有I型胶原蛋白且没有II型胶原蛋白。在第0周和第4周的组织学上，HUCM构建体定量地显示出比TMJ构建体含有更多的GAG。此外，HUCM构建体的细胞性在第0周高出55%，并且在4周后高出近两倍，尽管以相同的密度接种。HUCM构建体的生物合成水平的提高和更高的细胞性清楚地表明，HUCM干细胞在规定的条件下优于TMJ髁突软骨细胞。因此，HUCM干细胞可能是一个有吸引力的替代髁突软骨细胞的颞下颌关节组织工程应用。此外，鉴于HUCM干细胞的可用性和容易获得性，这些发现可能具有深远的影响，导致颅面和整形外科组织替代疗法的新发展。
The temporomandibular joint ( TMJ) presents many problems in modern musculoskeletal medicine. Patients who suffer from TMJ disorders often experience a major loss in quality of life due to the debilitating effects that TMJ disorders can have on everyday activities. Cartilage tissue engineering can lead to replacement tissues that could be used to treat TMJ disorders. In this study, a spinner flask was used for a period of 6 days to seed polyglycolic acid ( PGA) scaffolds with either TMJ condylar chondrocytes or mesenchymal- like stem cells derived from human umbilical cord matrix ( HUCM). Samples were then statically cultured for 4 weeks either in growth medium containing chondrogenic factors or in control medium. Immunohistochemical staining of HUCM constructs after 4 weeks revealed a strong presence of collagen I and minute amounts of collagen II, whereas TMJ constructs revealed little collagen I and no collagen II. The HUCM constructs were shown to contain more GAGs than the TMJ constructs quantitatively at week 0 and histologically at week 4. Moreover, the cellularity of HUCM constructs was 55% higher at week 0 and nearly twice as high after 4 weeks, despite being seeded at the same density. The increased level of biosynthesis and higher cellularity of HUCM constructs clearly demonstrates that the HUCM stem cells outperformed the TMJ condylar cartilage cells under the prescribed conditions. HUCM stem cells may therefore be an attractive alternative to condylar cartilage cells for TMJ tissue engineering applications. Further, given the availability and ease of obtaining HUCM stem cells, these findings may have far- reaching implications, leading to novel developments in both craniofacial and orthopaedic tissue replacement therapies.