Phosphorylation of tick-borne encephalitis virus NS5 protein
Phosphorylation of tick-borne encephalitis virus NS5 protein
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DOI：
10.1016/s0168-1702(96)01433-5
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发表时间：
1997-05-01
期刊：
影响因子：
5
通讯作者：
Kit, YY
中科院分区：
文献类型：
--
作者：
Morozova, OV;Tsekhanovskaya, NA;Kit, YY
最大的蜱传脑炎病毒（TBEV）非结构蛋白NS 5（100 kDa）被认为参与RNA复制。在[γ-P-32]ATP存在下，感染细胞提取物中的蛋白质被磷酸化，如十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和使用针对TBEV NS 5蛋白的单克隆抗体的蛋白质印迹分析所示。感染后早期阶段细胞提取物中NS 5的放射性标记高于感染后24小时。在Mg ~（2+）存在下，NS 5蛋白的免疫沉淀物与[γ-P-32]ATP孵育导致TBEV NS 5蛋白和免疫球蛋白的磷酸化。磷酸氨基酸分析表明，NS 5含有磷酸丝氨酸，但不含磷酸苏氨酸或磷酸酪氨酸。(C)1997年Elsevier Science B.V.
The largest tick-borne encephalitis virus (TBEV) non-structural protein NS5 (100 kDa) is believed to be involved in RNA replication. The protein is phosphorylated in infected cell extracts in the presence of [gamma-P-32]ATP, as shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis using monoclonal antibodies raised against TBEV NS5 protein. Radioactive labeling of NS5 in cellular extracts at an early stage post-infection is higher than at 24 h post-infection. Incubation of immunoprecipitates of NS5 protein with [gamma-P-32]ATP in the presence of Mg2+ resulted in the phosphorylation of TBEV NS5 protein and of immunoglobulins. Phosphoamino acid analysis demonstrated that NS5 contains phosphoserine, but not phosphothreonine, or phosphotyrosine. (C) 1997 Elsevier Science B.V.