Cyclical expression of GnRH and GnRH receptor mRNA in lymphoid organs
Cyclical expression of GnRH and GnRH receptor mRNA in lymphoid organs
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DOI：
10.1159/000054306
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发表时间：
1998-02-01
影响因子：
4.1
通讯作者：
Wilder, RL
中科院分区：
文献类型：
--
作者：
Jacobson, JD;Crofford, LJ;Wilder, RL
已知促性腺激素释放激素 (GnRH) 具有直接免疫调节作用，我们之前已经证明，GnRH 类似物的施用可独立于性腺类固醇的作用来调节小鼠狼疮的表达，我们推测 GnRH 在免疫细胞水平上发挥直接作用，已在淋巴细胞上鉴定出 GnRH 受体，因为 GnRH 和 GnRH 受体 (GnRH-R) 的表达随动情而变化。在下丘脑和垂体水平上，我们推测淋巴组织中也可能存在类似的周期性。在本报告中，我们使用竞争性逆转录 PCR 来定量小鼠整个动情周期中淋巴器官中 GnRH 和 GnRH-R mRNA 的表达。我们证明，脾脏中 GnRH-R mRNA 的表达模式与同一小鼠垂体中的模式以及先前报道的大鼠垂体中的模式非常一致，与发情后期早晨相比，发情前期下午的表达水平显着增加，胸腺中 GnRH-R mRNA 的表达也有类似的模式。其受体的表达模式，与发情后期的早晨相比，发情前期下午的表达水平显着增加。其他体外研究表明，GnRH 和雌二醇均显着增加免疫细胞中 GnRH-R mRNA 的表达。这些发现支持 GnRH 在免疫系统中的积极作用。
Gonadotropin-releasing hormone (GnRH) is known to possess dirct immunomodulatory effects, We have previously demonstrated that the administration of GnRH analogues modulates the expression of murine lupus independently of effects on gonadal steroids, We speculate that GnRH exerts direct actions at the level of the immune cells, GnRH receptors have been identified on lymphocytes, Because GnRH and GnRH receptor (GnRH-R) expression varies with the estrous cycle at the levels of the hypothalamus and pituitary, we speculated that similar cyclicity might be demonstrable in lymphoid tissue, In this report, we used competitive reverse transcription PCR to quantitate the expression of GnRH and GnRH-R mRNA in lymphoid organs throughout the estrous cycle in mice. We demonstrate that the pattern of expression of GnRH-R mRNA in spleen agrees closely with the pattern in the pituitaries of the same mice and the pattern previously reported in the rat pituitary, with significantly increased levels of expression seen on the afternoon of proestrus compared to the morning of metestrus, A similar pattern is seen with GnRH-R mRNA expression in the thymus, Furthermore, we show that the expression of GnRH mRNA in both thymus and spleen agrees closely with the pattern of expression of its receptor, with significantly increased levels of expression seen on the afternoon of proestrus compared to the morning of metestrus, Additional in vitro studies demonstrate that both GnRH and estradiol significantly increase the expression of GnRH-R mRNA in immune cells, These findings support an active role for GnRH in the immune system.