Methodologies for the determination of adenosine phosphates.
Methodologies for the determination of adenosine phosphates.
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DOI：
10.1016/0003-2697(78)90399-8
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发表时间：
1978-07
影响因子：
2.9
通讯作者：
W. D. Bostick;B. S. Ausmus
中科院分区：
文献类型：
--
作者：
W. D. Bostick;B. S. Ausmus
三磷酸腺苷（ATP）和其他磷酸腺苷化合物的测定是微生物生物量和微生物活性的有用指标。比较了测定这些化合物的各种光度法。对众所周知的己糖激酶-葡萄糖-6-磷酸脱氢酶的ATP过程进行了修改，该过程允许非常敏感的ATP + ADP的动力学测量（~ 3.1 pmol）。该过程利用磷酸烯醇丙酮酸和丙酮酸激酶再生ATP（前一反应中的底物），因此是一个扩增系统。较长的反应时间和产生的NADPH的荧光测量将进一步提高该程序的灵敏度。最后，一种简单、快速的远紫外分光光度法，以前被认为是对单磷酸腺苷的特异性，被证明可以提供对二磷酸腺苷（ADP）和ATP的定量估计。
Measures of adenosine triphosphate (ATP) and other adenosine phosphate compounds are useful indices of microbial biomass and microbiological activity. Various photometric procedures for the determination of these compounds are compared. A modification in the well-known hexokinase — glucose-6-phosphate dehydrogenase procedure for ATP, which permits a very sensitive kinetic measurement of ATP + ADP (∼3.1 pmol), is presented. The procedure utilizes phosphoenol pyruvate and pyruvate kinase to regenerate ATP (substrate in the preceding reaction) and thus is an amplification system. Longer reaction times and fluorimetric measurement of NADPH produced would further enchance the sensitivity of the procedure. Finally, a simple, rapid far-uv spectrophotometric procedure, previously believed to be specific for adenosine monophosphates, is demonstrated to provide quantitative estimates for adenosine diphosphate (ADP) and ATP as well.