Phylogenetic relationships within the genus Tetrahymena inferred from the cytochrome c oxidase subunit 1 and the small subunit ribosomal RNA genes
Phylogenetic relationships within the genus Tetrahymena inferred from the cytochrome c oxidase subunit 1 and the small subunit ribosomal RNA genes
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DOI：
10.1016/j.ympev.2008.09.017
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发表时间：
2008-12-01
影响因子：
4.1
通讯作者：
Lynn, Denis H.
中科院分区：
文献类型：
--
作者：
Chantangsi, Chitchai;Lynn, Denis H.
四膜虫纤毛虫的系统发育关系的细节仍然没有得到解决，尽管有丰富的历史调查与核基因序列和其他字符。我们研究了所有可用的四膜虫和其他三种四膜虫纤毛虫，并推断出它们的共生关系，使用几乎完整的线粒体细胞色素c氧化酶亚基1（cox1）和小亚基（SSU）rRNA基因序列。推测的系统发育关系表明四膜虫属是单系的。这三个“经典”的形态学和生态学为基础的分组是并系的。SSUrRNA基因组学证实了先前建立的australis和borealis分组，以及9个核糖体。然而，这九个核糖组没有得到很好的支持。利用cox 1基因，基于该基因推导的遗传学发现了12个支持性很好的群，称为coxisets，它们主要对应于9个核糖体。这项研究表明，cox1解决最近的四膜虫的系统发育的效用，而SSU rRNA基因提供了更深层次的属内的系统发育关系的决议。(C)2008年爱思唯尔公司All rights reserved.
Details of the phylogenetic relationships among tetrahymenine ciliates remain unresolved despite a rich history of investigation with nuclear gene sequences and other characters. We examined all available species of Tetrahymena and three other tetrahymenine ciliates, and inferred their phylogenctic relationships using nearly complete mitochondrial cytochrome c oxidase subunit 1 (cox1) and small subunit (SSU) rRNA gene sequences. The inferred phylogenies showed the genus Tetrahymena to be monophyletic. The three "classical" morphology-and-ecology-based groupings are paraphyletic. The SSUrRNA phylogeny confirmed the previously established australis and borealis groupings, and nine ribosets. However, these nine ribosets were not well supported. Using cox1 gene, the deduced phylogenies based on this gene revealed 12 well supported groupings, called coxisets, which mostly corresponded to the nine ribosets. This study demonstrated the utility of cox1 for resolving the recent phylogeny of Tetrahymena, whereas the SSU rRNA gene provided resolution of deeper phylogenetic relationships within the genus. (C) 2008 Elsevier Inc. All rights reserved.