Sensitization to Inhaled Ryegrass Pollen by Collateral Priming in a Murine Model of Allergic Respiratory Disease
Sensitization to Inhaled Ryegrass Pollen by Collateral Priming in a Murine Model of Allergic Respiratory Disease
复制标题
过敏性呼吸道疾病小鼠模型中通过并行启动对吸入黑麦草花粉致敏
DOI：
--
复制
发表时间：
2010
影响因子：
2.8
通讯作者：
J. Ceuppens
中科院分区：
文献类型：
--
作者：
P. Cadot;I. Meyts;J. Vanoirbeek;B. Vanaudenaerde;D. Bullens;J. Ceuppens
背景：哮喘小鼠模型需要在呼吸道暴露之前通过注射来启动动物。我们的目标是开发一种小鼠模型，模拟低剂量吸入过敏原暴露后人类过敏性疾病的进展。方法：小鼠腹膜内注射卵清蛋白 (OVA)，然后反复暴露于 OVA、黑麦草 (Lolium perenne) 花粉提取物或同时接触两者的气雾剂。通过测量特异性血清抗体、对黑麦草花粉提取物攻击的呼吸反应以及攻击后肺细胞因子的产生来评估对黑麦草花粉蛋白的敏感性。结果：单独吸入黑麦草花粉提取物不会导致过敏。然而，对吸入黑麦草花粉蛋白的过敏确实发生在通过腹腔注射对 OVA 敏感，然后同时暴露于吸入黑麦草花粉提取物和 OVA 的小鼠中。通过黑麦草花粉特异性 IgG1 和 IgE 抗体的产生以及用黑麦草花粉提取物刺激气道后诱导气道炎症和肺部 Th2 细胞因子 mRNA 转录物来确定 T 和 B 细胞启动。通过比较第 56 天和第 86 天的抗体模式，可以在免疫印迹中观察到 IgE/IgG1 对不同黑麦草花粉蛋白的反应逐渐扩散。结论：当气道同时暴露于动物已经过敏的另一种过敏原时，低剂量吸入性过敏原暴露会导致过敏。该模型可以帮助揭示呼吸道过敏性疾病发生和进展的机制。
Background: Mouse models of asthma suffer from the necessity to prime the animals by injections before respiratory exposure. Our aim was to develop a mouse model that mimics the progression of human allergic disease upon low-dose inhaled allergen exposure. Methods: Mice were primed intraperitoneally to ovalbumin (OVA) before they were exposed repeatedly to aerosols of either OVA, ryegrass (Lolium perenne) pollen extract, or both concomitantly. The sensitization to ryegrass pollen proteins was evaluated by measurement of specific serum antibody, by the respiratory response to a challenge with ryegrass pollen extract and by lung cytokine production after challenge. Results: Inhalation of ryegrass pollen extract alone did not result in sensitization. Sensitization to inhaled ryegrass pollen proteins, however, did occur in mice that had been sensitized to OVA by intraperitoneal injections and were then exposed to inhaled ryegrass pollen extract and OVA simultaneously. T and B cell priming was ascertained by ryegrass pollen-specific IgG1 and IgE antibody production and by induction of airway inflammation and of Th2 cytokine mRNA transcripts in the lungs upon airway challenge with ryegrass pollen extract. A progressive spread of the IgE/IgG1 response to different ryegrass pollen proteins could be visualized in immunoblots by comparing antibody patterns at day 56 and 86. Conclusions: Low-dose inhalatory allergen exposure results in sensitization when airways are exposed at the same time to another allergen to which the animals are already sensitized. This model can help to unravel the mechanisms that underlie the development and progression of respiratory allergic diseases.
登录
查看更多内容
DOI：
10.1164/ajrccm.156.3.9606031
发表时间：
1997-09-01
影响因子：
24.7
作者：
Hamelmann, E;Schwarze, J;Gelfand, EW
通讯作者：
Gelfand, EW
Th2 表型传播的诱导和抑制：对儿童哮喘的影响。
DOI：
10.4049/jimmunol.174.9.5864
发表时间：
2005
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hayashi,Tomoko;Gong,Xing;Rossetto,Cyprian;Shen,Carol;Takabayashi,Kenji;Redecke,Vanessa;Spiegelberg,Hans;Broide,David;Raz,Eyal
通讯作者：
Raz,Eyal
HIV gp120 与 CD4 和/或 mAb 的非共价复合物可增强 gp120 特异性 T 克隆的激活，并为抗 CD4 抗体的产生提供分子间帮助。
影响因子：
4.4
作者：
Manca,F;Seravalli,E;Valle,MT;Fenoglio,D;Kunkl,A;LiPira,G;Zolla-Pazner,S;Celada,F
通讯作者：
Celada,F
影响因子：
14.2
作者：
Goplen, Nicholas;Karim, Zunayet;Liang, Qiaoling;Gorska, Magdalena M.;Rozario, Sadee;Guo, Lei;Alam, Rafeul
通讯作者：
Alam, Rafeul