Growth‐stimulating effect of catecholamines on rat aortic smooth muscle cells in culture
Growth‐stimulating effect of catecholamines on rat aortic smooth muscle cells in culture
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儿茶酚胺对培养的大鼠主动脉平滑肌细胞的生长刺激作用
DOI：
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发表时间：
1983
影响因子：
5.6
通讯作者：
J. Boissel
中科院分区：
文献类型：
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作者：
N. Blaes;J. Boissel
观察肾上腺素对体外培养的大鼠动脉平滑肌细胞(SMC)增殖的影响。每天添加到培养基中的肾上腺素对生长有显著的刺激作用。该效应随时间延长而增强，并呈剂量依赖关系。浓度为1010M的−刺激作用最强，作用72h后刺激作用最强。较高浓度(10−，3M)肾上腺素对系膜细胞有毒性作用。当SMC通过剥夺血清而保持静止时，随后添加肾上腺素需要血清来显著促进生长。这种生长刺激作用随着血清浓度的增加而增加(从0.1%到10%)。所有受试的肾上腺素能激动剂都能刺激SMC的生长，其活性由大到小依次为：去甲肾上腺素、肾上腺素和异丙肾上腺素。最后，SMC对儿茶酚胺的这种有丝分裂反应是特异的，因为它可以被肾上腺素能阻滞剂阻断，在这一连接中，酚妥拉明比心得安更有效。结果提示，肾上腺素和其他儿茶酚胺可能是至少在大鼠的主动脉SMC的生长因子，主要通过肾上腺素受体发挥作用。
The effect of epinephrine was tested on the proliferation of rat arterial smooth muscle cells (SMC) in secondary cultures. Epinephrine added daily to the culture medium caused a striking stimulation of growth. The effect increased with time and was dose‐dependent. Maximal stimulation was observed at a concentration of 1010−5 M and after 72 hours. At higher concentrations (10−3 M) epinephrine exhibited toxic effects on SMC. When SMC were maintained quiescent by deprivation of serum, the subsequent addition of epinephrine required serum to significantly enhance growth. This growth stimulation increased with serum concentration (from 0.1 % to 10%). All the adrenergic agonists tested were found to stimulate SMC growth, with an activity classified by decreasing order as follows: norepinephrine > epinephrine > isoproterenol. Finally, this mitogenic response of SMC to catecholamines was specific since it could be blocked by adrenergic blocking agents, phentolamine being more efficient than propranolol in that connection. The results suggest that epinephrine and other catecholamines may act as growth factors for aortic SMC, at least in rat, mostly through adrenoreceptors.
血清低密度脂蛋白对培养的主动脉平滑肌细胞具有促有丝分裂作用。
影响因子：
5.3
作者：
Fless,GM;Kirchhausen,T;Fischer-Dzoga,K;Wissler,RW;Scanu,AM
通讯作者：
Scanu,AM